Articles

Promising bioactive compounds from the marine environment and their potential effects on various diseases

The marine environment hosts a wide variety of species that have evolved to live in harsh and challenging conditions. Marine organisms are the focus of interest due to their capacity to produce biotechnologica...

Authors: Akash Karthikeyan, Abey Joseph and Baiju G. Nair

Prevalence of Helicobacter pylori infection in patients with chronic hepatitis C

Association between Helicobacter pylori (H. pylori) and chronic hepatitis C (CHC) still remains controversial. This work is concerned with assessing the potential role of H. pylori in the progression of hepatitis...

Authors: Abdelfattah M. Attallah, Mohamed S. Albannan, Mohamed F. Ghaly, Sally E. Sallam, Mahmoud M. Amer and Attia A. Attia

The enhancement of microbial fuel cell performance by anodic bacterial community adaptation and cathodic mixed nickel–copper oxides on a graphene electrocatalyst

Although microbial fuel cells (MFCs) represent a promising technology for capturing renewable energy from wastewater, their scaling-up is significantly limited by a slow-rate cathodic oxygen reduction reaction...

Authors: Dena Z. Khater, R. S. Amin, M. O. Zhran, Zeinab K. Abd El-Aziz, Mohamed Mahmoud, Helmy M. Hassan and K. M. El-Khatib

Novel methylation mark and essential hypertension

Essential hypertension (EH) is an important risk factor for various cardiovascular, cerebral and renal disorders. It is a multi-factorial trait which occurs through complex interplay between genetic, epigeneti...

Authors: Mayank Chaudhary

Immunity evasion: consequence of the N501Y mutation of the SARS-CoV-2 spike glycoprotein

Authors: Henrietta Onyinye Uzoeto, Judith Nnedimkpa Ajima, Amarachukwu Vivian Arazu, Glory Omini Ibiang, Samuel Cosmas and Olanrewaju Ayodeji Durojaye

Phylogenetic analysis of phytochrome A gene from Lablab purpureus (L.) Sweet

Phytochromes are the best characterized photoreceptors that perceive Red (R)/Far-Red (FR) signals and mediate key developmental responses in plants. It is well established that photoperiodic control of floweri...

Authors: Stuti Krishna, Kaushal Modha, Vipulkumar Parekh, Ritesh Patel and Digvijay Chauhan

Molecular characterization of myelin basic protein a (mbpa) gene from red-bellied pacu (Piaractus brachypomus)

Myelin basic protein (MBP) is one of the most important structural components of the myelin sheaths in both central and peripheral nervous systems. MBP has several functions including organization of the myeli...

Authors: Juan Sebastian Cruz-Méndez, María Paula Herrera-Sánchez, Ángel Enrique Céspedes-Rubio and Iang Schroniltgen Rondón-Barragán

Missense variants in the TNFA epitopes and their effects on interaction with therapeutic antibodies—in silico analysis

Tumor necrosis factor alpha (TNFA) is an important cytokine that influences multiple biological processes. It is one of the key mediators of acute and chronic systemic inflammatory reactions and plays a centra...

Authors: Tamim Ahsan and Abu Ashfaqur Sajib

Correction to: In silico structural homology modelling of EST073 motif coding protein of tea Camellia sinensis (L)

An amendment to this paper has been published and can be accessed via the original article.

Authors: K. H. T. Karunarathna, N. H. K. S. Senathilake, K. M. Mewan, O. V. D. S. J. Weerasena and S. A. C. N. Perera

Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

Silver nanoparticles (AgNPs) are the most widely used nanomaterial in agricultural and environmental applications. In this study, the impact of AgNPs solutions at 20 mg/L, 40 mg/L, 80 mg/L, and 160 mg/L on cel...

Authors: May Labeeb, Abdelfattah Badr, Soliman A. Haroun, Magdy Z. Mattar and Aziza S. El-kholy

The role of BCL9 genetic variation as a biomarker for hepatitis C-related hepatocellular carcinoma in Egyptian patients

Hepatocellular carcinoma (HCC) is considered one of the most common cancers related to mortality around the world, and susceptibility is related with genetic, lifestyle, and environmental factors. Copy number ...

Authors: Eman Abd El Razek Abbas, Ahmed Barakat Barakat, Mohamed Hassany and Samar Samir Youssef

Identification in silico and expression analysis of a β-1-4-endoglucanase and β-galactosidase genes related to ripening in guava fruit

Guava fruit softening is a crucial process during ripening and this process involves a number of enzymes that modifies the cell wall. Two of the enzymes that regulate this process are (a) the β-1, 4-endoglucan...

Authors: Mario A. Mejía-Mendoza, Cristina Garcidueñas-Piña, José S. Padilla-Ramírez, Ruth E. Soria-Guerra and José Francisco Morales-Domínguez

Response surface methodology mediated optimization of Lignin peroxidase from Bacillus mycoides isolated from Simlipal Biosphere Reserve, Odisha, India

Lignin is a complex polymer of phenyl propanoid units found in the vascular tissues of the plants as one of lignocellulose materials. Many bacteria secrete enzymes to lyse lignin, which can be essential to eas...

Authors: Subhashree Rath, Manish Paul, Hemanta Kumar Behera and Hrudayanath Thatoi

Adaptive laboratory evolution triggers pathogen-dependent broad-spectrum antimicrobial potency in Streptomyces

In the present study, adaptive laboratory evolution was used to stimulate antibiotic production in a Streptomyces strain JB140 (wild-type) exhibiting very little antimicrobial activity against bacterial pathogens...

Authors: Dharmesh Harwani, Jyotsna Begani, Sweta Barupal and Jyoti Lakhani

Genetic epidemiology of autoinflammatory disease variants in Indian population from 1029 whole genomes

Autoinflammatory disorders are the group of inherited inflammatory disorders caused due to the genetic defect in the genes that regulates innate immune systems. These have been clinically characterized based o...

Authors: Abhinav Jain, Rahul C. Bhoyar, Kavita Pandhare, Anushree Mishra, Disha Sharma, Mohamed Imran, Vigneshwar Senthivel, Mohit Kumar Divakar, Mercy Rophina, Bani Jolly, Arushi Batra, Sumit Sharma, Sanjay Siwach, Arun G. Jadhao, Nikhil V. Palande, Ganga Nath Jha…

Assessment role of total phenols and flavonoids extracted from Pleurotus columbinus mushroom on the premature ovarian failure induced by chemotherapy in rats

Many species of mushroom contain an assortment of free radical scavengers (Phenolics and Flavonoids compounds) that have made them nutritionally beneficial and a source of expansion of drug production. In this...

Authors: Amal I. Hassan, Mona A. M. Ghoneim, Manal G. Mahmoud and Mohsen S. Asker

Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

The Pehuén or Monkey puzzle tree (Araucaria araucana) is an evergreen coniferous tree, which has been historically used for social, medicinal, and nutritional purposes. We have recently showed the value of A. ara...

Authors: María C. Gallia, Julieta Echeverri Del Sarto and Guillermina A. Bongiovanni

A plant-biotechnology approach for producing highly potent anti-HIV antibodies for antiretroviral therapy consideration

Despite a reduction in global HIV prevalence the development of a pipeline of new therapeutics or pre-exposure prophylaxis to control the HIV/AIDS epidemic are of high priority. Antibody-based therapies offer ...

Authors: Advaita Acarya Singh, Priyen Pillay, Lusisizwe Kwezi and Tsepo Lebiletsa Tsekoa

A highly efficient auxin-producing bacterial strain and its effect on plant growth

Various bacteria promote plant root growth in the rhizosphere, as a measure of securing and enlarging their ecological niche. These interactions are mediated by plant growth regulators (PGRs) such as auxin, an...

Authors: Seunghye Park, A-Leum Kim, Yoon-Kyung Hong, Ji-Hwan Shin and Se-Hwan Joo

Molecular marker for characterization of traditional and hybrid derivatives of Eleusine coracana (L.) using ISSR marker

Finger millet is the most important food grain in the world for its nutritional benefits. Finger millet is genetically and geographically diverse and widely spread in the African and Asian sub-continent. There...

Authors: Jayalakshmi Venkatesan, Vasuki Ramu, Thilaga Sethuraman, Chandrasekaran Sivagnanam and Ganesh Doss

Nitrogen deficiency regulates premature senescence by modulating flag leaf function, ROS homeostasis, and intercellular sugar concentration in rice during grain filling

Leaf senescence occurs in an age-dependent manner, but the rate and timing of leaf senescence may be influenced by various biotic and abiotic factors. In the course of stress, the function, composition, and di...

Authors: Shamsu Ado Zakari, Syed Hassan Raza Zaidi, Mustapha Sunusi and Kabiru Dawaki Dauda

Evaluation of the inhibitory potentials of selected compounds from Costus spicatus (Jacq.) rhizome towards enzymes associated with insulin resistance in polycystic ovarian syndrome: an in silico study

Polycystic ovary syndrome (PCOS) is a chronic endocrine disorder prevalent in premenopausal women and is characterized by a range of physiological and biochemical abnormalities which may include reproductive, ...

Authors: Fehintoluwa Joy Femi-Olabisi, Ahmed Adebayo Ishola, Opeyemi Faokunla, Anthonia Oluyemi Agboola and Benjamin Ayodipupo Babalola

Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

Gerbera jamesonii Bolus ex Hooker f. (African daisy) is listed among the top five most important ornamental plants in the global floricultural industry. To satisfy its demand, the floriculture industry relies on...

Authors: Saikat Gantait and Manisha Mahanta

Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation

Orthotropic liver transplantation (OLT) offers a therapeutic choice for hepatocellular carcinoma (HCC) patients. The poor outcome of liver transplantation is HCV recurrence. Several genome-wide associated stud...

Authors: Ghada M. Salum, Mai Abd el Meguid, Tawfeek H. Abelhafez, Eman Medhat, Ashraf O. Abdel Aziz and Reham Dawood

Salinity tolerance mechanisms and their breeding implications

The era of first green revolution brought about by the application of chemical fertilizers surely led to the explosion of food grains, but left behind the notable problem of salinity. Continuous application of...

Authors: Mandeep Singh, Usha Nara, Antul Kumar, Anuj Choudhary, Hardeep Singh and Sittal Thapa

Molecular docking and pharmacokinetic studies of phytocompounds from Nigerian Medicinal Plants as promising inhibitory agents against SARS-CoV-2 methyltransferase (nsp16)

Since the index case was reported in China, COVID-19 has led to the death of at least 4 million people globally. Although there are some vaccine cocktails in circulation, the emergence of more virulent variant...

Authors: Tolulope Peter Saliu, Haruna I. Umar, Olawale Johnson Ogunsile, Micheal O. Okpara, Noriyuki Yanaka and Olusola Olalekan Elekofehinti

Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars

Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, whic...

Authors: Valentina Di Pauli, Paola Daniela Fontana, Dalia Marcela Lewi, Arturo Felipe and Luis Ernesto Erazzú

Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)

Insulin-like growth factor 2 (IGF2) is one of three hormones that share high structural similarity to insulin. It is involved in several insulin-like growth-regulating and mitogenic activities. This study was con...

Authors: Dhafer A. Ali, Mohammed Baqur S. Al-Shuhaib, Golzar Farhadi, Fadhil R. Al-Kafajy, Tahreer M. Al-Thuwaini and Ali Esmailizadeh

Are single nucleotide polymorphisms rs7903146 and rs12255372 in transcription factor 7-like 2 gene associated with an increased risk for gestational diabetes mellitus in Egyptian women?

Genetic variants in the transcription factor 7-like 2 (TCF7L2) gene are related with type 2 diabetes (T2D) and gestational diabetes mellitus (GDM) in various populations, but there are not enough statistics regar...

Authors: Taghreed A. Shalabi, Khalda S. Amr and Mai M. Shaker

A novel model based on interleukin 6 and insulin-like growth factor II for detection of hepatocellular carcinoma associated with hepatitis C virus

The coexistence of cirrhosis complicates the early detection of hepatocellular carcinoma (HCC). Thus, novel biomarkers for HCC early detection are needed urgently. Traditionally, HCC detection is carried out b...

Authors: Mohamed M. Omran, Sara Mosaad, Tarek M. Emran, Fathy M. Eltaweel and Khaled Farid

Characterization of transcription factor MYB59 and expression profiling in response to low K⁺ and NO₃⁻ in indica rice (Oryza sativa L.)

Nitrogen and potassium are crucial supplements for plant development and growth. Plants can detect potassium and nitrate ions in soils and in like way, they modify root-to-shoot transport of these ions to adju...

Authors: Md. Qamrul Islam, Md. Nazmul Hasan, Hammadul Hoque, Nurnabi Azad Jewel, Md. Fahmid Hossain Bhuiyan and Shamsul H. Prodhan

Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes

Wild medicinal plants are suffering natural environmental stresses and habitat destruction. The genetic diversity evaluation of wild accessions and their in vitro raised genotypes using molecular markers, as w...

Authors: Abdelfattah Badr, Hanaa H. El-Shazly, Mahmoud Sakr, Mai M. Farid, Marwa Hamouda, Eman Elkhateeb and Hanan Syed Ahmad

The potential role of inhaled nitric oxide for postexposure chemoprophylaxis of COVID-19

Several vaccines have been fast-tracked in an attempt to decrease the morbidity and mortality of COVID-19. However, post-exposure prophylaxis has been overlooked in battling COVID-19.

Authors: Antoine AbdelMassih, Rafeef Hozaien, Meryam El Shershaby, Aya Kamel, Habiba-Allah Ismail, Mariem Arsanyous, Nadine El-Husseiny, Noha Khalil, Youstina Naeem and Raghda Fouda

The variation in promoter sequences of the Akt3 gene between cow and buffalo revealed different responses against mastitis

Serine/threonine kinase 3 (AKT3) is a protein-coding gene that is associated with several cattle immune diseases including different tumors and cancers. The objective of this study was to investigate the differen...

Authors: Farmanullah Farmanullah, Mostafa Gouda, Zhang Min, Xu Sutong, Mohib Ullah KaKar, Sami Ullah Khan, Muhammad Salim, Momen Khan, Zia ur Rehman, Hira Sajjad Talpur, Faheem Ahmed Khan, Nuruliarizki Shinta Pandupuspitasari and Zhang Shujun

In silico molecular and morphological analysis of rice blast resistant gene Pi-ta in Sri Lankan rice germplasm

Pi-ta is a major blast resistant gene, introgressed from indica rice varieties. In this study, diversity of the Pi-ta gene of 47 Sri Lankan rice accessions was studied by bioinformatics, and the results were vali...

Authors: Suvanthini Terensan, H. Nishadi S. Fernando, J. Nilanthi Silva, S. A. Chandrika N. Perera, Nisha S. Kottearachchi and O. V. D. S. Jagathpriya Weerasena

Isolation and identification of marine microbial products

The ocean is one of the world’s most important sources of bioactive chemicals in the marine environment. Microbiologists, ecologists, agronomists, taxonomists, and evolutionary biologists have been increasingl...

Authors: Sahar Saleh Mohamed, Sayeda Abdelrazek Abdelhamid and Radwa Hassaan Ali

Docking and ADMET studies for investigating the anticancer potency of Moscatilin on APC10/DOC1 and PKM2 against five clinical drugs

Moscatilin is a bibenzyl derivative (stilbenoid), mainly found in Dendrobium species. This plant-derived chemical is a potential cytotoxic anticancer drug that acts against different cancer types. The present stu...

Authors: Ipsita Pujari, Ritobrata Sengupta and Vidhu Sankar Babu

Investigation of promoter regions, motifs, and CpG islands in the regulation of gene expression in Trametes hirsuta strain 072

In silico analysis of transcription start sites, promoter regions, transcription factors and their binding sites, and CpG islands for the Trametes hirsuta strain 072 genome were performed to understand the regula...

Authors: Dinku Senbeta and Mulugeta Kebede

Hybridoma technology; advancements, clinical significance, and future aspects

Hybridoma technology is one of the most common methods used to produce monoclonal antibodies. In this process, antibody-producing B lymphocytes are isolated from mice after immunizing the mice with specific an...

Authors: Sanchita Mitra and Pushpa Chaudhary Tomar

Association of NOS3 (rs 2070744) and SOD2Val16Ala (rs4880) gene polymorphisms with increased risk of ESRD among Egyptian patients

Chronic kidney Failure (CKD), particularly End-Stage Renal Disease (ESRD), may be serious ill-health related to a high death rate. Uremic syndrome leads to increased oxidative stress, inflammation, and dyslipi...

Authors: Afaf Elsaid, Omnia Samir eid, Samy B. Said and Rasha F. Zahran

NAFLD mark: an accurate model based on microRNA-34 for diagnosis of non-alcoholic fatty liver disease patients

It remains essential for non-alcoholic fatty liver (NAFLD) patients, to develop a sensitive and specific diagnostic model. Data regarding the use of micro (mi)RNA-34 for NAFLD diagnosis are few. Routine clinic...

Authors: Amal A. Mohamed, Ahmed El-Demery, Eman Al-Hussain, Shroouk Mousa, Ahmed Abdel Halim, Sahar M. Mostafa, Reda S. Abdelghany, Seham M. Mahmoud, Mohammad A. Elkady, Khaled Raafat, Alshymaa A. Hassnine and Mohamed M. Omran

Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics

Penicillin G amidase/acylases from microbial sources is a unique enzyme that belongs to the N-terminal nucleophilic hydrolase structural superfamily. It catalyzes the selective hydrolysis of side chain amide/a...

Authors: Krishika Sambyal and Rahul Vikram Singh

Expression of acid cleavable Asp-Pro linked multimeric AFP peptide in E. coli

Difficult to express peptides are usually produced by co-expression with fusion partners. In this case, a significant mass part of the recombinant product falls on the subsequently removed fusion partner. On t...

Authors: Murad Mollaev, Artur Zabolotskii, Neonila Gorokhovets, Elena Nikolskaya, Maria Sokol, Andrey Tsedilin, Mariia Mollaeva, Margarita Chirkina, Timofey Kuvaev, Anna Pshenichnikova and Nikita Yabbarov

Role of “dual-personality” fragments in HEV adaptation—analysis of Y-domain region

Hepatitis E is a liver disease caused by the pathogen hepatitis E virus (HEV). The largest polyprotein open reading frame 1 (ORF1) contains a nonstructural Y-domain region (YDR) whose activity in HEV adaptatio...

Authors: Zoya Shafat, Anwar Ahmed, Mohammad K. Parvez and Shama Parveen

Transcriptomic in silico analysis of bovine Escherichia coli mastitis highlights its immune-related expressed genes as an effective biomarker

Mastitis is one of the major diseases causing economic loss to the dairy industry by reducing the quantity and quality of milk. Thus, the objective of this scientific study was to find new biomarkers based on ...

Authors: Farmanullah Farmanullah, Xianwei Liang, Faheem Ahmed Khan, Mohammad Salim, Zia ur Rehman, Momen Khan, Hira Sajjad Talpur, N. M. Schreurs, Mostafa Gouda, Sami Ullah Khan and Zhang Shujun

Physicochemical and molecular characterization of heavy metal–tolerant bacteria isolated from soil of mining sites in Nigeria

Mining for precious metals is detrimental to the composition of soil structure and microbial diversity distribution and is a health risk to human communities around the affected communities. This study was aim...

Authors: U. B. Ibrahim, A. H. Kawo, I. Yusuf and S. Yahaya

Transcriptome comparisons detect new genes associated with apoptosis of cattle and buffaloes preantral follicles

To develop new breeding technology to improve the breeding ability of bovine, it is the development trend to find the main reason for the occurrence of atresia in these organisms. Transcriptomes of small (100–...

Authors: Khairy Mohamed Zoheir, Ahmed Mohamed Darwish, Yang Liguo and Abdelkader E. Ashour

IL28B rs12979860 polymorphism and zinc supplementation affect treatment outcome and liver fibrosis after direct-acting antiviral hepatitis C therapy

Impact of interleukin 28B (IL28B) rs12979860 polymorphism on response to direct-acting antivirals agents in HCV genotype 4-infected patients is under investigation. Zinc may have an advantage in improvement of li...

Authors: Abdelfattah M. Attallah, Dalia Omran, Mohamed A. Abdelrazek, Mohamed Hassany, Sameh Saif, Aza Farid, Riham El Essawey, Muhammad Abdel Ghaffar, Marwa Aabdelghany and Ayman Yosry

Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

Soil pollution by heavy metals increases the bioavailability of metals like hexavalent chromium (Cr (VI)), subsequently limiting plant growth and reducing the efficiency of phytoremediation. Plant growth-promo...

Authors: Nabil Tirry, Aziza Kouchou, Bouchra El Omari, Mohamed Ferioun and Naïma El Ghachtouli

Gene introduction approaches in chloroplast transformation and its applications

Chloroplast is a type of plastid that is believed to be originated from ancestral cyanobacteria. Chloroplast besides being a major component for photosynthesis, also takes part in another major plant metabolis...

Authors: Asqwin Uthaya Kumar and Anna Pick Kiong Ling