Articles

Exploring the diversity of endophytic fungi and screening for their pullulanase-producing capabilities

Pullulanases are the significant industrial group in the 13 glycosyl hydrolases category, known as the α-amylases family. There are very few reports on pullulanase from fungal sources. Based on the above resea...

Authors: Bindu Naik, S. K. Goyal, Abhishek Dutt Tripathi and Vijay Kumar

IGF1R, IGFALS, and IGFBP3 gene copy number variations in a group of non-syndromic Egyptian short children

Insulin-like growth factor-1 (IGF-1) is required for normal intrauterine and postnatal growth, and this action is mediated through IGF1 receptor (IGF1R). IGF1R copy number variants (CNVs) can cause pre- and postn...

Authors: Islam M. Fadel, Moustafa H. Ragab, Ola M. Eid, Nivine A. Helmy, Hala T. El-Bassyouni and Inas Mazen

Metabolic profiling, in vitro propagation, and genetic assessment of the endangered rare plant Anarrhinum pubescens

Anarrhinum pubescens Fresen. (Plantaginaceae) is a rare plant, endemic to the Saint Catherine area, of South Sinai, Egypt. Earlier studies have reported the isolation of cytotoxic and anti-cholinesterase iridoid ...

Authors: Asmaa Abdelsalam, Ehab Mahran, Kamal Chowdhury and Arezue Boroujerdi

The keratinolytic bacteria Bacillus cytotoxicus as a source of novel proteases and feather protein hydrolysates with antioxidant activities

Argentina’s geothermal areas are niches of a rich microbial diversity. In 2020, species of Bacillus cytotoxicus were isolated for the first time from these types of pristine natural areas. Bacillus cytotoxicus st...

Authors: Ivana Cavello, Brenda Bezus and Sebastián Cavalitto

Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds

The discovery of potential, new cost-effective drug resources in the form of bioactive compounds from mushrooms is one way to control the resistant pathogens. In the present research, the fruiting bodies of fi...

Authors: Elham R. S. Soliman and Heba El-Sayed

Clonal hematopoiesis of indeterminate potential (CHIP) and cardiovascular diseases—an updated systematic review

Cardiovascular diseases (CVDs) are the leading cause of mortality in India. Residual risk exists in patients receiving optimal guideline-directed medical therapy. Possession of certain somatic mutations, at a ...

Authors: Nagendra Boopathy Senguttuvan, Vinodhini Subramanian, Vettriselvi Venkatesan, T. R. Muralidharan and Kavitha Sankaranarayanan

Anti-HIV reverse transcriptase plant polyphenolic natural products with in silico inhibitory properties on seven non-structural proteins vital in SARS-CoV-2 pathogenesis

Accessing COVID-19 vaccines is a challenge despite successful clinical trials. This burdens the COVID-19 treatment gap, thereby requiring accelerated discovery of anti-SARS-CoV-2 agents. This study explored th...

Authors: Von Novi O. de Leon, Joe Anthony H. Manzano, Delfin Yñigo H. Pilapil IV, Rey Arturo T. Fernandez, James Kyle Anthony R. Ching, Mark Tristan J. Quimque, Jay Carl M. Agbay, Kin Israel R. Notarte and Allan Patrick G. Macabeo

Screening and characterization of hypothetical proteins of Plasmodium falciparum as novel vaccine candidates in the fight against malaria using reverse vaccinology

Plasmodium falciparum is the most deadly and leading cause of morbidity and mortality in Africa. About 90% of all malaria deaths in the world today occur in Sub-Saharan Africa especially in children aged < 5 year...

Authors: Claire Aguttu, Brenda Apio Okech, Ambrose Mukisa and George William Lubega

Isolation and characterization of marine sponge–associated Streptomyces sp. NMF6 strain producing secondary metabolite(s) possessing antimicrobial, antioxidant, anticancer, and antiviral activities

Actinomycetes associated with marine sponge represent a promising source of bioactive compounds. Herein, we report the isolation, identification, and bioactivity evaluation of Streptomyces sp. NMF6 associated wit...

Authors: Nayer Mohamed Fahmy and Asmaa Mohamed Abdel-Tawab

Andrographolide binds to spike glycoprotein and RNA-dependent RNA polymerase (NSP12) of SARS-CoV-2 by in silico approach: a probable molecule in the development of anti-coronaviral drug

The SARS-CoV-2 belongs to Coronaviridae family infects host cells by the interaction of its spike glycoprotein and angiotensin-converting enzyme 2 (ACE 2) of host cells. Upon entry, the virus uses its RNA depe...

Authors: Lokanathan Srikanth and Potukuchi Venkata Gurunadha Krishna Sarma

Gene network analysis to determine the effect of hypoxia-associated genes on brain damages and tumorigenesis using an avian model

Hypoxia refers to the condition of low oxygen pressure in the atmosphere and characterization of response to hypoxia as a biological complex puzzle, is challenging. Previously, we carried out a comparative gen...

Authors: Hamed Kharrati-Koopaee, Esmaeil Ebrahimie, Mohammad Dadpasand, Ali Niazi, Rugang Tian and Ali Esmailizadeh

Isolation and characterization of salt-tolerant bacteria with plant growth-promoting activities from saline agricultural fields of Haryana, India

Soil salinity has been one of the biggest hurdles in achieving better crop yield and quality. Plant growth-promoting rhizobacteria (PGPR) are the symbiotic heterogeneous bacteria that play an important role in...

Authors: Arti Sharma, Kamal Dev, Anuradha Sourirajan and Madhu Choudhary

Evaluation of MLPA as a comprehensive molecular cytogenetic tool to detect cytogenetic markers of chronic lymphocytic leukemia in Egyptian patients

Chronic lymphocytic leukemia (CLL) is the most common form of adult leukemia. This disease is genetically heterogeneous, and approximately 85% of patients with CLL harbor chromosomal aberrations that are consi...

Authors: Ola M. Eid, Rania M. A. Abdel Kader, Lamiaa A. Fathalla, Amany H. Abdelrahman, Ahmed Rabea, Rana Mahrous and Maha M. Eid

Scaling-up production of cost-effective and eco-friendly bio-fertilizer and its application on Barley green fodder via IoT hydroponic system

Plant-associated microbes (endophytes) have a significant relationship to enhance plant growth and crop productivity by producing proficient bioactive metabolites. Since endophytes promoted plant growth either...

Authors: Mustafa Elsayed Abd Elsallam, Shahira Hussainy EL-Moslamy, Ahmed Abd El-Al and Hoda Farouk Zahran

Development of stable transgenic maize plants tolerant for drought by manipulating ABA signaling through Agrobacterium-mediated transformation

In crop plants, to cope up with the demand of food for rising population, revolutionary crop improvement programmes are being implemented for higher and higher yields. Abiotic stress, especially at flowering s...

Authors: Sridevi Muppala, Pavan Kumar Gudlavalleti, Kodandarami Reddy Malireddy, Sateesh Kumar Puligundla and Premalatha Dasari

ApoB gene polymorphism (rs676210) and its pharmacogenetics impact on atorvastatin response among Iraqi population with coronary artery disease

Drug response is below genetic influence, proven by the genetic variants. Pharmacogenetics trials are performed in many diseases, including coronary artery disease. This study was designed to determine the gen...

Authors: Shaimaa Y. Abdulfattah and Salwa J. Al-Awadi

In silico analysis of promoter region and regulatory elements of mitogenome co-expressed trn gene clusters encoding for bio-pesticide in entomopathogenic fungus, Metarhizium anisopliae: strain ME1

Pest control strategies almost entirely rely on chemical insecticides, which cause environmental problems such as biosphere deterioration and emergence of resistant pests. Bio-pesticide is an alternative appro...

Authors: Getachew Bantihun and Mulugeta Kebede

Comparison of matK and rbcL DNA barcodes for genetic classification of jewel orchid accessions in Vietnam

Jewel orchid is the common name of several orchid species which can be alike in morphological characteristics, but variable in medicinal properties. At present, two DNA barcode loci, namely, maturase K (matK) and...

Authors: Viet The Ho, Thi Kim Phuong Tran, Thi Thanh Tram Vu and Sasanti Widiarsih

Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans

Candida albicans is an opportunistic human fungal pathogen responsible for superficial and systemic life-threatening infections. Treating these infections is challenging as many clinical isolates show increased d...

Authors: Wasim Uddin, Darshan Dhabalia, S. M. Udaya Prakash and M. Anaul Kabir

Implications of targeted next-generation sequencing for bladder cancer: report of four cases

Bladder cancer is considered heterogeneous diseases with two major subgroups: non-muscle- invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). It is a major healthcare problem, and it is ...

Authors: Mohamed K. Khalifa, Noha M. Bakr, Amal Ramadan, Khaled M. Abd Elwahab, Esam Desoky, Amira M. Nageeb and Menha Swellam

Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems

Whilst traditional strategies to increase transfection efficiency of non-viral systems aimed at modifying the vector or the polyplexes/lipoplexes, biomaterial-mediated gene delivery has recently sparked increa...

Authors: Valeria Graceffa

Phytohormones: plant switchers in developmental and growth stages in potato

Potato is one of the most important food crops worldwide, contributing key nutrients to the human diet.

Authors: Abbas Saidi and Zahra Hajibarat

Immunofluorescence studies to dissect the impact of Cockayne syndrome A alterations on the protein interaction and cellular localization

Cockayne syndrome (CS), which was discovered by Alfred Cockayne nearly 75 years ago, is a rare autosomal recessive disorder characterized by growth failure, neurological dysfunction, premature aging, and other...

Authors: Amr Ghit

Molecular characterization of marine bacterial isolates of Visakhapatnam coast—efficacy in dye decolorization and bioremediation of cadmium

Microbial community is one of the diversified communities of the marine environment. Studies have shown that microorganisms isolated from the marine environment are metabolically active and have adapted to lif...

Authors: Teja Mandragutti, Muni Kumar Dokka, Bindiya Panchagnula and Sudhakar Godi

Multiplex CRISPR/Cas9-mediated genome editing of the FAD2 gene in rice: a model genome editing system for oil palm

Genome editing employing the CRISPR/Cas9 system has been widely used and has become a promising tool for plant gene functional studies and crop improvement. However, most of the applied CRISPR/Cas9 systems tar...

Authors: Bohari Bahariah, Mat Yunus Abdul Masani, Omar Abd Rasid and Ghulam Kadir Ahmad Parveez

A study on mutation points of GDF9 gene and their association with prolificacy in Egyptian small ruminants

Genetic variants of the GDF9 gene were considered to be the potent gene markers for improving fecundity traits in Egyptian sheep and goats. Also, these favorable gene variants could be applied in the breeding ...

Authors: Dalia M. Aboelhassan, Ahmed M. Darwish, Neama I. Ali, Inas S. Ghaly and Ibrahim M. Farag

Impact of circulating miRNA-373 on breast cancer diagnosis through targeting VEGF and cyclin D1 genes

Breast cancer (BC) is the common primary tumor among females. Hence, there is an urgent need to improve the early prediction and diagnosis of BC. For that reason, the object of the current study is to analyze ...

Authors: Noha M. Bakr, Magda Sayed Mahmoud, Reem Nabil, Hussein Boushnak and Menha Swellam

In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources

Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and develo...

Authors: Ahmed Madi Waheed Al-Mayahi

PPAR agonists as effective adjuvants for COVID-19 vaccines, by modifying immunogenetics: a review of literature

Several coronavirus vaccine have been fast-tracked to halt the pandemic, the usage of immune adjuvants that can boost immunological memory has come up to the surface. This is particularly of importance in view...

Authors: Antoine Fakhry AbdelMassih, Rahma Menshawey, Jumana H. Ismail, Reem J. Husseiny, Yousef M. Husseiny, Shenoda Yacoub, Aya Kamel, Rafeef Hozaien, Elaria Yacoub, Esraa Menshawey, Abanoub Abdelmalek, Ahmed Abouelazaem, Ahmed Elhatw, Ahmed Aboelmaaty, Alaaelrahman Shahib, Amany Mansour…

The immune response and protective efficacy of a potential DNA vaccine against virulent Pasteurella multocida

Pasteurella multocida is the main cause of several infections of farm animals, and the immunity gained from commercial vaccines is for the short term only and needs to be routinely administered, so work on new va...

Authors: Ahmed A. M. Yassein, Ayaat A. Teleb, Gamal M. Hassan and Zaki A. El Fiky

Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

The use of natural preservatives became of great interest; good examples of these natural preservation agents are plant peels. The use of plant peels has dual benefits; first is their antimicrobial activity ag...

Authors: Safynaz Magdy Hanafy, Yasser Mohamed Abd El-Shafea, Waleed Diaeddeen Saleh and Hayam Mohamed Fathy

β-Sitosterol differentially regulates key metabolites for growth improvement and stress tolerance in rice plants during prolonged UV-B stress

Elevated ultraviolet-B (UV-B) radiation is potentially deleterious to many organisms specifically crop plants and has become a global challenge. Rice is an exceptionally important staple food which is grown wo...

Authors: Raheel Shahzad, Mohamed Ewas, Putri Widyanti Harlina, Shahid Ullah Khan, Pan Zhenyuan, Xinhui Nie and Elsayed Nishawy

Correction to: New insights on key genes involved in drought stress response of barley: gene networks reconstruction, hub, and promoter analysis

Authors: Seyedeh Mehri Javadi, Zahra-Sadat Shobbar, Asa Ebrahimi and Maryam Shahbazi

The challenge of prognostic markers in acute pancreatitis: internist’s point of view

Acute pancreatitis, the most frequent hospitalization reason in internal medicine ward among gastrointestinal diseases, is burdened by high mortality rate. The disease manifests mainly in a mild form, but abou...

Authors: Ombretta Para, Lorenzo Caruso, Maria Teresa Savo, Elisa Antonielli, Eleonora Blasi, Fabio Capello, Tiziana Ciarambino, Lorenzo Corbo, Armando Curto, Margherita Giampieri, Lucia Maddaluni, Giacomo Zaccagnini and Carlo Nozzoli

Synthesis and characterizations iron oxide carbon nanotubes nanocomposite by laser ablation for anti-microbial applications

Environmental contamination by microbes is a major public health concern. A damp environment is one of the potential sources for microbe proliferation. Smart synthesis nanocatalytic coatings on surfaces, food,...

Authors: Moatasem Al-Salih, Syakirah Samsudin and Siti Suri Arshad

Defining the molecular pathology and consequent phenotypes in Egyptian HB patients

Hemophilia B (HB) (also known as Christmas disease) is a rare X-linked recessive disorder characterized by spontaneous or prolonged hemorrhages caused by mutations in Factor 9 (F9) gene leading to deficient or de...

Authors: Ghada Y. El-Kamah, Rehab M. Mosaad, Mohamed B. Taher and Khalda S. Amr

Green synthesis of silk sericin-embedded silver nanoparticles and their antibacterial application against multidrug-resistant pathogens

The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver nanoparticles (AgNPs) have been synthesized by natural products such as star...

Authors: Md. Abdullah Al Masud, Hamid Shaikh, Md. Shamsul Alam, M. Minnatul Karim, M. Abdul Momin, M. Ariful Islam and G. M. Arifuzzaman Khan

Study of lipase producing gene in wheat – an in silico approach

Lipases (EC 3.1.1.3) catalyze the hydrolysis of oil into free fatty acids and glycerol forming the 3rd largest group of commercialized enzymes. Plant lipases grab attention recently because of their specificit...

Authors: Shradha Rani, Priya Kumari, Raju Poddar and Soham Chattopadhyay

Secondary metabolites and biodiversity of actinomycetes

The ability to produce microbial bioactive compounds makes actinobacteria one of the most explored microbes among prokaryotes. The secondary metabolites of actinobacteria are known for their role in various ph...

Authors: Manal Selim Mohamed Selim, Sayeda Abdelrazek Abdelhamid and Sahar Saleh Mohamed

Molecular characterization of a novel β-defensin isoform from the red-toothed trigger fish, Odonus niger (Ruppel, 1836)

The concern regarding a post-antibiotic era with increasing drug resistance by pathogens imposes the need to discover alternatives for existing antibiotics. Antimicrobial peptides (AMPs) with their versatile t...

Authors: S. Neelima, K. Archana, P. P. Athira, M. V. Anju, V. V. Anooja, I. S. Bright Singh and Rosamma Philip

Production of polyclonal antibody against the recombinant PirB^vp protein of Vibrio parahaemolyticus

Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirA^vp and PirB^vp encoded in pVA1 plasmid. The polyclonal antibod...

Authors: Ngoc-Diem Duong, Khai-Hoan Nguyen-Phuoc, Kim-Yen Thi Do, Nguyet-Thu Thi Nguyen, Thuoc Linh Tran and Hieu Tran-Van

An immunoinformatics approach for the design of a multi-epitope vaccine targeting super antigen TSST-1 of Staphylococcus aureus

TSST-1 is a secretory and pyrogenic superantigen that is being responsible for staphylococcal mediated food poisoning and associated clinical manifestations. It is one of the main targets for the construction ...

Authors: Harish Babu Kolla, Chakradhar Tirumalasetty, Krupanidhi Sreerama and Vijaya Sai Ayyagari

Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

Trachyspermum ammi is one of the key medicinal plant species with many beneficial properties. Thymol is the most important substance in the essential oil of this plant. Thymol is a natural monoterpene phenol with...

Authors: Masoumeh Nomani and Masoud Tohidfar

Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought

The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, inc...

Authors: Ailin Beznec, Paula Faccio, Daniel J. Miralles, Leonor G. Abeledo, Cecilia Decima Oneto, María de Belén Garibotto, Germán Gonzalez, Federico Moreyra, Matías Elizondo, Mónica Ruíz, Dalia Lewi, Eduardo Blumwald, Berta Llorente, Antonio Díaz Paleo and Ezequiel Bossio

Clinical impact of PTEN methylation status as a prognostic marker for breast cancer

Aberrant DNA methylation of phosphatase and tensin homolog (PTEN) gene has been found in many cancers. The object of this study was to evaluate the clinical impact of PTEN methylation as a prognostic marker in...

Authors: Amal Ramadan, Maha Hashim, Amr Abouzid and Menha Swellam

Confirmation of “pre-plasmolysis mediated ex-osmosis hypothesis” to obtain shoot bud morphogenesis in Catharanthus roseus

The antineoplastic herb, Catharanthus roseus is a classified high-value low-volume medicinal herb which is in global attention of scientific research for modulation of its monoterpenoid indole alkaloids (MIA) pat...

Authors: Vyoma Mistry, Abhishek Sharma and Ajay Kumar Mathur

Genetic diversity and population structure of date palms (Phoenix dactylifera L.) in Ethiopia using microsatellite markers

Date palm tree (Phoenix dactylifera L.) is a perennial monocotyledonous plant belonging to the Arecaceae family, a special plant with extraordinary nature that gives eminent contributions in agricultural sustaina...

Authors: Workia Ahmed, Tileye Feyissa, Kassahun Tesfaye and Sumaira Farrakh

Immunocontraceptive potential of a GnRH receptor-based fusion recombinant protein

The management of stray dog population has been of utmost importance due to their overpopulation, increase in dog bites incidence, and rabies. Contraceptive vaccines, a non-surgical alternative to spaying and ...

Authors: Nathaniel Philip Sandam, Dhamodhar Prakash and Prashanth Thimmareddy

Analgesic and anti-inflammatory potential of Lupeol isolated from Indian traditional medicinal plant Crateva adansonii screened through in vivo and in silico approaches

Lupeol, a triterpene bioactive compound isolated from Indian traditional plant Crateva adansonii acted as promising and alternative anti-inflammatory agent to treatments of diseases related to inflammation. The i...

Authors: Thirumalaisamy Rathinavel, Subramanian Ammashi and Gnanendra Shanmugam

High-throughput screening of natural compounds and inhibition of a major therapeutic target HsGSK-3β for Alzheimer’s disease using computational approaches

Alzheimer’s disease is a leading neurodegenerative disease worldwide and is the 6th leading cause of death in the USA. AD is a very complex disease and the drugs available in the market cannot fully cure it. T...

Authors: Rohit Shukla and Tiratha Raj Singh