Table 5 Results of PCR simulation using FastPCR
From: Bioinformatics study of phytase from Aspergillus niger for use as feed additive in livestock feed
Code | Primer | Position | % anneal | Tm (°C) | Ta (°C) | Amplikon size (product) |
|---|---|---|---|---|---|---|
48.2 | Forward | 1–19 | 100 | 62.4 | 70 | 1404 |
|  | Reverse | 1381–1404 | 100 | 64 |  |  |
92 | Forward | 1–19 | 100 | 62.5 | 70 | 1515 |
|  | Reverse | 1492–1515 | 100 | 64 |  |  |
110 | Forward | 1–24 | 100 | 66.2 | 72 | 1506 |
|  | Reverse | 1483–1506 | 100 | 64.0 |  |  |