Table 3 Primer design results with Primer3 + (P3) and oligocalculator (Olc)
From: Bioinformatics study of phytase from Aspergillus niger for use as feed additive in livestock feed
Code | Sequence | Base | Tm (°C) | GC content (%) | |||
|---|---|---|---|---|---|---|---|
P3 +  | Olc | P3 +  | Olc | ||||
(….. primer forward…..) | |||||||
48.2 | 5′ATGGGCGTCTCTGCTGTTC3′ | 19 bp | 61.4 | 59.5 | 57.9 | 58 | |
92 | 5′ATGGGTGTCTCTGCCGTTC3′ | 19 bp | 61.1 | 59.5 | 57.9 | 58 | |
110 | 5′ATGGGCGTCTCTGCTGTTCTACTT3′ | 24 bp | 64.7 | 65.2 | 50 | 50 | |
(……primer reverse…..) | |||||||
48.2 | 5′CTAAGCAAAACACTCCGCCCAATC3′ | 24 bp | 66.9 | 65.2 | 50 | 50 | |
92 | 5′CTAAGCAAAACACTCCGCCCAATC3′ | 24 bp | 66.9 | 65.2 | 50 | 50 | |
110 | 5′CTAAGCAAAACACTCCGCCCAATC3′ | 24 bp | 66.9 | 65.2 | 50 | 50 | |