Characterization, antimicrobial and antitumor activity of superoxide dismutase extracted from Egyptian honeybee venom (Apis mellifera lamarckii)

Journal of Genetic Engineering and Biotechnology

Table 3 Effect of various inhibitors on BVSOD

Reagent	Final concentration (mM)	Inhibition (%)
Control	––-	0.0
Potassium cyanide (KCN)	2.0	83.6
Potassium cyanide (KCN)	5.0	98.3
Hydrogen peroxide (H₂O₂)	2.0	79.4
Hydrogen peroxide (H₂O₂)	5.0	95.1
Sodium azide (NaN₃)	2.0	26.8
Sodium azide (NaN₃)	5.0	37.6
Sodium dodecyl sulfate (SDS)	2.0	10.6
Sodium dodecyl sulfate (SDS)	5.0	13.3
Ethylenediaminetetraacetic acid (EDTA)	2.0	43.1
Ethylenediaminetetraacetic acid (EDTA)	5.0	51.7
DL-Dithiothreitol (DTT)	2.0	28.3
DL-Dithiothreitol (DTT)	5.0	36.7
β-Mercaptoethanol	2.0	34.5
β-Mercaptoethanol	5.0	41.8
1,10-Phenanthroline	2.0	20.4
1,10-Phenanthroline	5.0	28.6
Phenylmethylsulfonyl fluoride (PMSF)	2.0	17.3
Phenylmethylsulfonyl fluoride (PMSF)	5.0	19.6
Iodoacetamide	2.0	30.4
Iodoacetamide	5.0	39.2
Potassium dichromate (K₂Cr₂O₇)	2.0	52.8
Potassium dichromate (K₂Cr₂O₇)	5.0	71.3