Table 2 Effect of different inhibitors on the purified camel thrombin
Inhibitor | Concentration | Camel thrombin inhibition % |
|---|---|---|
No inhibitor | –- | 0.0 |
Benzamidine HCl | 5 mM | 13.5 |
Cysteine | 5 mM | 5.5 |
EDTA | 5 mM | 11.0 |
EGTA | 5 mM | 14.5 |
β-Mercaptoethanol | 5 mM | 35.0 |
PMSF | 2 mM | 93.0 |
Soybean trypsin inhibitor | 5 mM | 38.7 |
Camel tick salivary gland thrombin inhibitor | 2.5 µM | 97.0 |