Structural and functional characterization of a hypothetical protein in the RD7 region in clinical isolates of Mycobacterium tuberculosis — an in silico approach to candidate vaccines

Journal of Genetic Engineering and Biotechnology

Table 2 Comparison of the immunological properties of NHP1 protein with positive and negative controls

Prediction servers	NHP1	Positive control			Negative control
Prediction servers	NHP1	Rv3875	Rv3874	Rv1886c	Rv3260c	Rv0685	Rv1777
VaxiJen	0.450	0.557	0.782	0.584	0.356	0.398	0.370
AntigenPro	0.765	0.891	0.923	0.859	0.101	0.257	0.264
AllerTop	Non-allergen	Probable allergen	Non-allergen	Non-allergen	Non-allergen	Probable allergen	Non-allergen
AllergenFP	Non-allergen	Probable allergen	Non-allergen	Non-allergen	Non-allergen	Probable allergen	Non-allergen
ToxinPred	Nontoxic	Toxic	Nontoxic	Nontoxic	Toxic	Nontoxic	Non-toxic
VirulentPred	Virulent	Virulent	Virulent	Virulent	Virulent	Non-virulent	Virulent
HLAPred	Potential vaccine candidate	Potential vaccine candidate	Potential vaccine candidate	Potential vaccine candidate	Potential vaccine candidate	Can cause autoimmune disease	Potential vaccine candidate