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Journal of Genetic Engineering and Biotechnology
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Molecular diversity of exotic durian (Durio spp.) germplasm: a case study of Kalimantan, Indonesia

Journal of Genetic Engineering and Biotechnology volume 20, Article number: 39 (2022) Cite this article

Abstract

Background

Durian of Indonesia, specifically Durio zibethinus, is a potential agricultural commodity for domestic and international markets. However, its quality is still less competitive or significantly lower to fulfill the export market, compared to a similar one from other countries. This study aimed to determine and analyze the genetic diversity and relationship of the exotic durian (Durio spp.) germplasm originally from Kalimantan, Indonesia, using the rbcL marker.

Results

Based on this marker, the durian germplasm has a low genetic diversity (π%=0.24). It may strongly correspond with the variability sites or mutation present in the region. In this case, the rbcL region of the durian germplasm has generated 23 variable sites with a transition/transversion (Ti/Tv) bias value of 1.00. However, following the phylogenetic and principal component analyses, this germplasm is separated into four main clades and six groups, respectively. In this case, D. zibethinus was very closely related to D. exleyanus. Meanwhile, D. lowianus and D. excelsus were the farthest. In further analysis, 29 durians were very closely related, and the farthest was shown by Durian Burung (D. acutifolius) and Kalih Haliyang (D. kutejensis) as well as Pampaken Burung Kecil (D. kutejensis) and Durian Burung (D. acutifolius) with a divergence coefficient of 0.011. The Pearson correlation analysis confirms that 20 pairs of individual durians have a strong relation, shown by, e.g., Maharawin Hamak and Durian Burung as well as Mantuala Batu Hayam and Durian Burung Besar.

Conclusion

While the durian has a low genetic diversity, the phylogenetic analyses revealed that this germplasm originally from Kalimantan, Indonesia, shows unique relationships. These findings may provide a beneficial task in supporting the durian genetic conservation and breeding practices in the future, locally and globally.

Background

Durian, specifically Durio zibethinus, is a potentially valuable agricultural commodity for domestic and international markets [1]. Even it is a prospective export commodity today. For example, Indonesia, one of the world’s biggest durian producers, was able to sell this fruit to numerous other countries, including several Middle Eastern countries with a total value of 232,000 USD in 2020 [2]. Similarly, this country has generated over 1.19 million metric tons of durian in the same year [3]. However, compared to a similar commodity from two adjacent nations, i.e., Thailand and Malaysia, the quality of Indonesian durians is still less competitive (significantly lower) to fulfill the export market [4]. As a result, various strategic plans must be included in the breeding program to improve the quality of durian fruit, particularly.

Germplasm collection is a vital component of plant breeding program success or the development of new superior cultivars [5]. Around 18 of the world’s 27 durian species, including their wild relatives, have been discovered in Indonesia. There are even 16 species that are considered endemic, and the Kalimantan is the Indonesian island with the highest durian genetic diversity [6]. According to Uji [7], the nine edible durian species present in this region with delicious flesh taste and unique fruit characteristics, including D. dulcis, D. excelsus, D. grandiflorus, D. graveolens, D. kutejensis, D. lowianus, D. oxleyanus, D. testudinarum, and D. zibethinus. In addition, these durians have other advantages, such as being resistant to diseases, especially patch canker, and having a high tolerance for environmental challenges, such as acid soil [6]. As a result, the germplasm is useable as a parent in a breeding effort.

Germplasm characterization is also crucial to aiding the durian plant breeding initiative or generating new superior cultivars [5]. However, durian germplasm and its relatives have only been characterized using morphological markers so far [1]. While these markers have advantages, they are frequently multigenic and heavily impacted by environmental factors [8]. Furthermore, morphological ones are inefficient since they can only be applied to mature plants, wait for the generative phase (flowers), and are time-consuming to apply [1]. The genetic diversity and relationships of durians have already been studied using various molecular markers, including RAPD [1, 9, 10], SSR, and ISSR [11, 12]. However, because these markers are highly subjective, the study is less precise [13]. According to Wu et al. [14], these markers are also poor consistency, limited repeatability, or complicated operation limit their effectiveness.

This study aimed to determine and analyze the genetic diversity and relationship of the exotic durian (Durio spp.) germplasm originally from Kalimantan, Indonesia, using the rbcL marker. Following Moura et al. [15], this marker shows high universality and amplification success. Also, this marker has generated a relatively high-quality output and unbias sequence data [13, 16]. So it is useful for discriminating Angiosperms or germplasm with close genetic relationships [17]. In partial, this marker has been applied for various plant germplasm, such as Oryza sativa [18], Amorphophallus [19], Flacourtia inermis [20], and Ilex [21]. As a result, the findings of this study can be applied to help future durian germplasm breeding initiatives, both locally and globally.

Methods

Plant samples

In this study, we have used a total of eighteen samples of durian (Durio spp.) germplasm, excluding an outgroup (Table 1). Most of the durian samples were collected from South Kalimantan, Indonesia, using a purposive sampling method (Fig. 1). Meanwhile, an outgroup (Bombax ceiba) was obtained from the GenBank database.

Table 1 List of exotic durian (Durio spp.) germplasm used in this study, including their origin, GenBank accession number, and rbcL sequence length
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Fig. 1
figure 1

Map of Kalimantan, Indonesia, showing sampling locations where eighteen durians were collected and used in this study. The list of durian samples and their complete origin are shown in Table 1

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DNA assay

The DNAs were extracted from the durian leaves using a combined commercial kit from Molecular Research Center Inc., USA (DNAZol@Direct) and Geneaid Biotech Ltd., Taiwan (GP100). The genetic materials were then quantified using a UV-VIS spectrophotometer (NanoVue, GE Healthcare, UK) and amplified by a pair of rbcL primers, namely rbcL-F (5′-ATGTCACCACAAACAGAGACTAAAGC-3′) and rbcL-R (5′-GTAAAATCAAGTCCACC RCG-3′) [22]. Amplification was employed in the total volume reaction of 25 μL, consisting of 2 μL DNA template (20 ng), 1 μL for each primer (0.2 μmol), and 22 μL of MyTaq HS Red Mix (Bioline, UK). This reaction was setting with the following conditions: initial denaturation at 94 °C for 5 min; followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 48 °C for 30 s, and extension at 72 °C for 45 s; and a final extension at 72 °C for 7 min [18]. The amplicon (DNA product) was then separated on 2% agarose gel electrophoresis, including a 1X TBE buffer solution and DNA stain (GelRed, Biotium Inc., USA), and observed on UV transilluminator. The DNA product was then purified and sequenced by 1st Base Ltd., Malaysia, using the Sanger method, bi-directionally. All sequence (rbcL) targets were deposited in GenBank with accession numbers of MZ479678–MZ479695 (Table 1).

Data analysis

The rbcL sequences of durian were refined manually to a reconstructed consensus using the MEGA-X software [23]. These sequences were then aligned using Clustal-Omega [24] and MultAlin [25]. The genetic diversity of this germplasm was determined using the nucleotide diversity index (π%) with the following categories: 0.1–0.4 is low, 0.5–0.7 is moderate, and 0.8–2.00 is high [26]. The phylogenetic analysis was done by maximum likelihood (ML) and neighbor-joining (NJ) methods, with the assistance of MEGA-X [23]. The internal node of each phylogenetic tree was evaluated by the bootstrap method (1000 replicates) [27]. The genetic relationship was also determined using the principal component analysis (PCA) by the MVSP ver. 3.1 [28]. The AMOVA and evolutionary divergence among sequences were analyzed using the Kimura 2-parameter model [29]. In addition, the F-statistics at the molecular level were calculated among species (populations), and their significance was also tested by a permutation procedure, using 2000 permutations. These analyses were conducted by Arlequin [30]. The Pearson correlation (r) analysis, by the criteria of weak (r ≤ 0.35), moderate (r = 0.36–0.67), and strong (r > 0.68), was finally applied to confirm the differences in genetic structure between durian samples [31].

Results

The rbcL region of durians and its genetic diversity

The durian rbcL region was successfully amplified. The amplification results show that this region has a size of approximately 650 bp (Fig. 2). After sequencing, each durian (Durio spp.) sample had a different rbcL sequence length, ranging from 527–578 bp (Table 2). The multiple sequence alignment is presented in Fig. 3. Based on Table 2, this region has 44.39% of GC content, 23 variable sites, five Parsimony informative sites, six singleton sites, and 0.51 transition/transversion bias values. Table 3 shows detailed information on the Parsimony informative site’s position on the rbcL region of the durian germplasm. In this case, only two mutations were present in this region, namely substitution-transition and substitution-transversion (Table 3) or no indels therein (Table 2). Furthermore, following this region, the durian germplasm has a nucleotide diversity (π%) of 0.24, with the AMOVA shown in Table 4. Following Table 4, at inter-species, the durian germplasm has a lower variation (5.62%) than the intra-species level (94.38%).

Fig. 2
figure 2

Electrophoresis profile showing PCR products of exotic durian germplasm generated by the rbcL marker. The name of each durian sample is shown in Table 1

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Table 2 Genetic information of rbcL sequences of durian (Durio spp.) germplasm
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Fig. 3
figure 3

Multiple sequence alignment of rbcL of exotic durian (Durio spp.) germplasm, showing several mutational events, i.e., transition (green closed rectangle) and transversion (blue closed rectangle)

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Table 3 Parsimony informative sites on the rbcL sequences of durian (Durio spp.) germplasm
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Table 4 The AMOVA for durian (Durio spp.) germplasm, both inter- and intra-species levels
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Genetic relationship and divergence

The durian (Durio spp.) germplasm from Kalimantan, Indonesia, shows unique relationships. This uniqueness lies in the number and composition of durian members in each clade formed. In general, following the maximum likelihood (ML) and neighbor-joining (NJ) methods, this germplasm is grouped into four main clades (Figs. 4 and 5, respectively). In this case, the first clade (I) is the largest, composed of nine durian germplasm for ML and ten for NJ. Clade II is the next largest consisting of five durian samples for ML and four for NJ. Clades III and IV consisted of two individuals, both ML and NJ. Interestingly, most of the durian samples were consistent in the same clade, both for ML and NJ, except for Durian Si Japang (D. zibethinus), which belongs to clade II in ML and clade I in NJ (Table 5).

Fig. 4
figure 4

Genetic relationship of exotic durian (Durio spp.) germplasm revealed by maximum likelihood (ML) and bootstrap analyses for 1000 replicates

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Fig. 5
figure 5

Genetic relationship of exotic durian (Durio spp.) germplasm revealed by Neighbor-Joining (NJ) and bootstrap analyses for 1000 replicates

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Table 5 Grouping of durian (Durio spp.) germplasm based on ML and NJ methods
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The PCA has generated differences in germplasm grouping. In this case, the durians have separated into six groups (Fig. 6), where group I was a largest and compose by six durians, i.e., Pampaken, Pampaken Burung Kecil, Malutu, Kura-Kura, Lahung Alang, and Si Japang. Meanwhile, other groups have consisted of two (IV and V) and three (II, III, and VI) members only (Fig. 6).

Fig. 6
figure 6

Grouping of exotic durian (Durio spp.) germplasm revealed by principal component analysis (PCA) method

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The divergence analysis (Table 6) showed that by species group, D. zibethinus was very closely related to D. exleyanus. Meanwhile, the farthest shows by D. lowianus and D. excelsus. Overall, the divergence analysis (Table 7) show a relationship between all samples (individuals), where 29 durian pairs are very closely related, and the farthest shown by Durian Burung (D. acutifolius) and Kalih Haliyang (D. kutejensis), and Pampaken Burung Kecil (D. kutejensis) with Durian Burung (D. acutifolius) as well, at a divergence coefficient of 0.011. The Pearson correlation analysis confirms that twenty pairs of individual durians have a strong relation (Fig. 7), for example, between Maharawin Hamak and Durian Burung as well as Mantuala Batu Hayam and Durian Burung Besar (Fig. 7).

Table 6 Genetic divergence among durian species (Durio spp.) used in this study
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Table 7 Genetic divergence among all exotic durian (Durio spp.) germplasm used in this study
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Fig. 7
figure 7

Pearson correlation coefficient among exotic durian (Durio spp.) germplasm based on the rbcL region

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Discussion

The rbcL region of the durian

The ribulose-1, 5-bisphosphate carboxylase/oxygenase, or rbcL, is a functional gene in the chloroplast genome engaged primarily in plant photosynthesis [32]. This gene is found in the chloroplast genome’s large single-copy (LSC) region and exhibits high similarity across plant germplasm [33]. According to Singh and Banerjee [34], this gene has a 600–800 nucleotide intergenic spacer. The rbcL gene contains around 1400 nucleotides that code for the large subunit protein, and the length varies significantly among flowering plants or Angiosperm [35].

In this study, the rbcL region of durians has different lengths, ranging from 527 to 578 bp (Table 2). These differences, both partial and complete, have been reported by several researchers. For example, Kumekawa et al. [36] have reported that durian (D. zibethinus) has a partial rbcL of 250 bp, and Amandita et al. [37] about 500 bp. In complete, this germplasm has the rbcL sequence of 1428 bp [38].

Further, a new DNA barcoding motif was discovered in the multiple sequence alignment of the rbcL of durians, in which a conserved region is introduced by polymorphism or other mutational events (Fig. 3). Based on Table 2, the rbcL durians of Kalimantan showed 23 variable sites or mutational events, and all are substitutions (transition-transversion), and no indels are present. According to Clegg [39], complete codon insertions/deletions are occasionally found in the gene, demonstrating a conservative pattern of nucleotide replacement. In general, grasses and other plant species such as Orchidales, Liliales, Bromeliales, and Arecales have a >5-fold differential in rbcL substitution rate [39].

According to Dong et al. [40], this gene represents distinctions in molecular evolution mode and tempo in angiosperms, monocotyledons, Gramineae, and Elymus. In another study, the inter/intrageneric levels of rbcL were highly efficient in Cornaceae, Cupressaceae, Ericaceae, and Graniaceae [34]. The rbcL gene evolved more quickly in annual plants, particularly in the Asteridae and Poaceae families, and was dubbed “most morphologically advanced forms” in these families [41].

Genetic diversity and its benefits

In this study, exotic durian (Durio spp.) germplasm originally from Kalimantan, Indonesia, has a low genetic diversity, shown by nucleotide diversity (π%) of 0.24 (Table 2). The low level of genetic diversity may be attributed to a combination of founder effects and subsequent bottlenecks encountered in its short domesticated history [42]. While the founder effect is a ubiquitous domestication bottleneck, millennia of cultivation and dissemination into new habitats have provided a considerable opportunity in selecting novel diversity in most crops [42].

Referred to Teixeira and Huber [43], low genetic diversity is often interpreted as an indicator of inbreeding depression and increased genetic drift. In other words, inbreeding, genetic drift, restricted gene flow, and small population size contribute to a genetic diversity reduction. Accordingly, populations lacking genetic diversity often exhibit an increased extinction rate [44]. Ujvari et al. [45] also reported that a decline in genetic diversity is linked to an increased risk of inbreeding depression, resulting in decreased growth rate, fertility, fecundity, and offspring viability, as well as in increased vulnerability to pathogens. Furthermore, a loss of genetic diversity would harm individual fitness with increased susceptibility to disease and parasites [44] and limits a population’s ability to respond to threats in reduced long- and short-term survival of endangered species [46].

Compared to other studies with similar markers used, durian (Durio spp.) germplasm from this region has a high diversity. For example, tidal swamp rice (Oryza sativa) shows a genetic diversity of 0.086. According to Teixeira and Huber [43], high levels of genetic diversity are beneficial to promoting population survival and guaranteeing the adaptive potential of natural populations in the face of rapidly changing environmental pressures. These principles are reflected in strategies such as genetic rescue, where the genetic diversity of a threatened or endangered population is increased by facilitating gene flow from a population with high levels of diversity [43].

However, emerging genetic diversity strongly correlated with the polymorphic or mutation found in a target region. According to Frankham et al. [47], genetic diversity and mutational events are two things that are related. In this study, the rbcL region of the durian germplasm has generated 23 variable sites with a transition/transversion (Ti/Tv) bias value of 1.00 (Table 2). Multiple alignments revealed that transversion is more than transition (Fig. 3 and Table 3). Guo et al. [48] have reported that the first mutation is a more frequent encounter in this sequence and has higher regulatory effects than transitions. However, a pattern of the last mutation is favored several times over transversions is commonly occur in molecular evolution [49, 50].

Regardless of the presence of mutations in the rbcL sequence of durians, genetic diversity is essential for plant genetic resources conservation, breeding practices, and preventing genetic basis erosion of breeding populations [14]. For these purposes, examining genetic diversity is essential in managing threatened species or taxa [46]. According to Teixeira and Huber [43], conservation genetic practice rests on the assumption that measured levels of diversity provide a direct indicator of the degree to which genetic factors contribute to the risk of extinction. For crop improvement, genetic diversity is beneficial for parental selection [51] or selecting parents with genetically divergent [14]. In this context, determining populations with a high level of genetic diversity will become a valuable resource for broadening the genetic base or gene pool of germplasm, as this enables the identification of superior alleles for several traits [51].

Following the AMOVA (Table 4), the durian germplasm has a higher variation (94.38%) at the intra-species level than the inter-species one (5.62%). It means that the future durian breeding program can be oriented to outcrossing, as was done by Hariyati et al. [10] and Prihatini et al. [9]. According to Uji [6], several wild durian species, except D. zibethinus, have potential genes that can be incorporated into this program, such as being resistant to diseases and having a high tolerance for environmental challenges.

Genetic relationship and divergence

The phylogenetic study or genetic relationships is also beneficial for plant genetic conservation and breeding practices [14]. For the first program, this study can be applied in inferring species and their evolutionary history, including species delimitation, genetic differentiation, and gene flow [52]. In other words, this information is given the objective metrics for conservation purposes in the past evolution history, genetic status of species in the present time, and management program for future ones [52]. For the second or last purposes, information of this relationship is usable in predicting the genetic diversity of the offspring when individuals or populations cross [5].

In this study, the durian (Durio spp.) germplasm from Kalimantan, Indonesia, shows unique relationships, mainly based on the number and composition of durian members in each clade or group formed. In general, following the maximum likelihood (ML) and neighbor-joining (NJ) methods, this germplasm is grouped into four main clades (Figs. 4 and 5, respectively). According to the PCA, this germplasm was separated into six groups (Fig. 6). Interestingly, both for ML and NJ, most of the durian samples were consistent in the same clade, except for Durian Si Japang (D. zibethinus), which belongs to Clade II in ML and Clade I in NJ (Table 5). Briefly, these phylogenetic trees (Figs. 4 and 5) and grouping illustrated the closeness and distant relationship between the samples.

The divergence analysis (Table 6) showed that by species group, D. zibethinus was very closely related to D. exleyanus. Meanwhile, the farthest shows by D. lowianus and D. excelsus. By ITS and ndhF markers, Nyffeler and Baum [53, 54] reported a close relationship between D. zibethinus and D. oxleyanus. Such relationships were also stated by Santoso et al. [55] using RFLP, Santoso et al. [12] by microsatellite, and Santoso et al. [56] with ITS.

However, within individuals (Table 7), 29 durian pairs are very closely related, and the farthest shown by Durian Burung (D. acutifolius) and Kalih Haliyang (D. kutejensis), and Pampaken Burung Kecil (D. kutejensis) with Durian Burung (D. acutifolius) as well, at a divergence coefficient of 0.011. Following the Pearson correlation analysis, only 20 pairs of individual durians have a strong relation, for example, Maharawin Hamak and Durian Burung as well as Mantuala Batu Hayam and Durian Burung Besar (Fig. 7). According to Acquaah [5], crossing individuals with distant relationships may generate high genetic diversity in the offspring. Conversely, crossing individuals with very close related may result in offspring with a low or narrow genetic diversity. In general, crossing individuals with a very close relationship is tends to avoid, as inbreeding occurs in the offspring [57]. Thus, our results are essential in supporting the future durian genetic conservation and breeding practices.

Conclusion

Following the rbcL region, the exotic durian (Durio spp.) germplasm originally from Kalimantan, Indonesia, has a low genetic diversity (π%=0.24). However, following the phylogenetic and principal component analyses, this germplasm is separated into four main clades and six groups, respectively. In this case, D. zibethinus is very closely related to D. exleyanus. Meanwhile, D. lowianus and D. excelsus are the farthest. Individually, 29 durians were very closely related, and the was farthest shown by Durian Burung (D. acutifolius) and Kalih Haliyang (D. kutejensis) as well as also Pampaken Burung Kecil (D. kutejensis) and Durian Burung (D. acutifolius) with a divergence coefficient of 0.011. The Pearson correlation analysis confirms that 20 pairs of individual durians have a strong relation, shown by (e.g.) Maharawin Hamak and Durian Burung, also Mantuala Batu Hayam and Durian Burung Besar. Our results may provide a fundamental paradigm in supporting the durian genetic conservation and breeding practices in the future, locally and globally.

Availability of data and materials

The datasets used and analyzed in the present study are presented in the article, and the rbcL sequences of this germplasm were deposited in GenBank with accession numbers.

Abbreviations

ITS:

Internal transcribed spacer

ML:

Maximum likelihood

NJ:

Neighbor-joining

PCA:

Principal component analysis

RFLP:

Restriction fragment length polymorphism

References

  1. Mursyidin DH, Daryono BS (2016) Genetic diversity of local durian (Durio zibethinus Murr.) cultivars of South Kalimantan’s province based on RAPD markers. In: AIP Conference Proceedings

    Google Scholar 

  2. Rizaty MA (2021) National production of durian [Produksi durian nasional]. https://databoks.katadata.co.id/datapublish/2021/06/23/produksi-durian-di-indonesia-menurun-pada-2020

    Google Scholar 

  3. Statista Research Department (2021) Production of durian in Indonesia 2011-2020. https://www.statista.com/statistics/706504/production-of-durian-in-indonesia/

    Google Scholar 

  4. Durian Harvests Indonesia (2021) Global durian production. https://www.durianharvestsindonesia.com/production/

    Google Scholar 

  5. Acquaah G (2007) Principles of plant genetics and breeding. Blackwell Publishing, Oxford

    Google Scholar 

  6. Uji T (2005) Species diversity and genetic resources of Durio (Durio spp.) in Indonesia [Keanekaragaman jenis dan sumber plasma nutfah Durio (Durio spp.) di Indonesia]. Buletin Plasma Nutfah 11:28–33

    Google Scholar 

  7. Uji T (2004) Species diversity, genetic resources, and potential of the indigenous fruits in Kalimantan [Keanekaragaman jenis, plasma nutfah, dan potensi buah-buahan asli Kalimantan]. Bio SMART 6:117–125

    Google Scholar 

  8. Jiang G-L (2013) Molecular markers and marker-assisted breeding in plants. In: Plant breeding from laboratories to fields. InTech Open Ltd., London, pp 44–83

  9. Prihatini R, Ihsan F, Indriyani NLP (2016) Genomic profiling of F1 hybrids of durian (Durio zibethinus) revealed by RAPD-PCR. J Hort Res 24:69–76. https://doi.org/10.1515/johr-2016-0022

    Article  Google Scholar 

  10. Hariyati T, Kusnadi J, Arumingtyas EL (2013) Genetic diversity of hybrid durian resulted from cross-breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs). Amer J Mol Biol 03:153–157. https://doi.org/10.4236/ajmb.2013.33020

    Article  Google Scholar 

  11. Ho VT, Ho MD, Tran TL (2020) Characterizing genetic variation of two popular durians (Durio zibethinus L.) varieties in southern Vietnam by using ISSR markers. Biosci Res 17:3040–3049

    Google Scholar 

  12. Santoso PJ, Granitia A, Indriyani NLP, Pancoro A (2016) Loci analysis and diversity of durian (Durio sp.) germplasm based on microsatellite markers [Analisis lokus dan keragaman sumber daya genetik durian (Durio sp.) berdasarkan marka mikrosatelit]. J Hort 26:9–20

    Article  Google Scholar 

  13. Lee SC, Wang CH, Yen CE, Chang C (2017) DNA barcode and identification of the varieties and provenances of Taiwan’s domestic and imported made teas using ribosomal internal transcribed spacer 2 sequences. J Food Drug Anal 25:260–274. https://doi.org/10.1016/j.jfda.2016.06.008

    Article  Google Scholar 

  14. Wu F, Ma S, Zhou J et al (2021) Genetic diversity and population structure analysis in a large collection of white clover (Trifolium repens L.) germplasm worldwide. PeerJ 9:1–17. https://doi.org/10.7717/peerj.11325

    Article  Google Scholar 

  15. Moura CCDM, Brambach F, Bado KJH et al (2019) Integrating DNA barcoding and traditional taxonomy for the identification of dipterocarps in remnant lowland forests of Sumatra. Plants 8:1–14. https://doi.org/10.3390/plants8110461

    Article  Google Scholar 

  16. Singh J, Kakade DP, Wallalwar MR et al (2017) Evaluation of potential DNA barcoding loci from plastid genome: intraspecies discrimination in rice (Oryza species). Intl J Curr Microbiol Appl Sci 6:2746–2756. https://doi.org/10.20546/ijcmas.2017.605.308

    Article  Google Scholar 

  17. Hollingsworth PM, Li DZ, van der Bank M, Twyford AD (2016) Telling plant species apart with DNA: from barcodes to genomes. Philos Trans R Soc B Biol Sci 371. https://doi.org/10.1098/rstb.2015.0338

  18. Mursyidin DH, Nazari YA, Badruzsaufari, Masmitra MRD (2021) DNA barcoding of the tidal swamp rice (Oryza sativa) landraces from South Kalimantan, Indonesia. Biodiv 22:1593–1599. https://doi.org/10.13057/biodiv/d220401

    Article  Google Scholar 

  19. Mursyidin DH, Hernanda MA (2021) Phylogenetic positions of three Amorphophallus species natively growing in the Meratus Mountains, South Kalimantan, Indonesia. Biodiversitas 22:2821–2828. https://doi.org/10.13057/biodiv/d220546

    Article  Google Scholar 

  20. Akilabindu K (2019) Genomic analysis of chloroplast matK and rbcL gene from Flacourtia inermis Roxb for plant DNA barcoding. GSC Biol Pharma Sci 9:065–071. https://doi.org/10.30574/gscbps.2019.9.2.0177

    Article  Google Scholar 

  21. Yao X, Tan YH, Yang JB et al (2019) Exceptionally high rates of positive selection on the rbcL gene in the genus Ilex (Aquifoliaceae). BMC Evol Biol 19:1–13. https://doi.org/10.1186/s12862-019-1521-1

    Article  Google Scholar 

  22. Gholave AR, Pawar KD, Yadav SR et al (2017) Reconstruction of molecular phylogeny of closely related Amorphophallus species of India using plastid DNA marker and fingerprinting approaches. Physiol Mol Biol Plants 23:155–167. https://doi.org/10.1007/s12298-016-0400-0

    Article  Google Scholar 

  23. Kumar S, Stecher G, Li M et al (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096

    Article  Google Scholar 

  24. Sievers F, Barton G, Higgins DG (2020) Multiple sequence alignments. In: Baxevanis A (ed) Bioinformatics. Wiley, New Jersey

    Google Scholar 

  25. Mitchell C (1993) MultAlin: multiple sequence alignment. Cabios Soft Rev 9:614–615

    Google Scholar 

  26. Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases (molecular evolution/mitochondrial DNA/nucleotide diversity). PNAS 76:5269–5273

    Article  Google Scholar 

  27. Lemey P, Salemi M, Vandamme AM (2009) The phylogenetic handbook: a practical approach to phylogenetic analysis and hypothesis testing, second. Cambridge University Press, Cambridge

    Book  Google Scholar 

  28. Kovach W (1999) MVSP-a multivariate statistical Package for Windows, ver. 3.1. Kovach Computing Services, Pentraeth, p 137

    Google Scholar 

  29. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  Google Scholar 

  30. Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x

    Article  Google Scholar 

  31. Taylor R (1990) Interpretation of the correlation coefficient: a basic review. J Diagn Med Sonogr 6:35–39. https://doi.org/10.1177/875647939000600106

    Article  Google Scholar 

  32. Liu L, Zhao B, Zhang Y, Wang J (2012) Adaptive evolution of the rbcL gene in Brassicaceae. Biochem Syst Ecol 44:13–19. https://doi.org/10.1016/j.bse.2012.04.007

    Article  Google Scholar 

  33. Dong W, Cheng T, Li C et al (2014) Discriminating plants using the DNA barcode rbcLb: an appraisal based on a large data set. Mol Ecol Resour 14:336–343. https://doi.org/10.1111/1755-0998.12185

    Article  Google Scholar 

  34. Singh J, Banerjee S (2018) Utility of DNA barcoding tool for conservation and molecular identification of intraspecies of rice genotypes belonging to Chhattisgarh using rbcL and matK gene sequences. Plant Arch 18:69–75

    Google Scholar 

  35. CBOL Plant Working Group (2009) A DNA barcode for land plants. PNAS 106:12794–12797

    Article  Google Scholar 

  36. Kumekawa Y, Kilmaskossu M, Mori M et al (2014) Changes in plant species during succession in a sago forest. Amer J Plant Sci 05:3526–3534. https://doi.org/10.4236/ajps.2014.524369

    Article  Google Scholar 

  37. Amandita FY, Rembold K, Vornam B et al (2019) DNA barcoding of flowering plants in Sumatra, Indonesia. Ecol Evol 9:1858–1868. https://doi.org/10.1002/ece3.4875

    Article  Google Scholar 

  38. Alverson WS, Karol KG, Baum DA et al (1998) Circumscription of the Malvales and relationships to other Rosidae: evidence from rbcL sequence data. Amer J Bot 85:876–887. https://doi.org/10.2307/2446423

    Article  Google Scholar 

  39. Clegg MT (1993) Chloroplast gene sequences and the study of plant evolution. PNAS 90:363–367

    Article  Google Scholar 

  40. Dong ZZ, Fan X, Sha LN et al (2013) Phylogeny and molecular evolution of the rbcL gene of St genome in Elymus sensu lato (Poaceae: Triticeae). Biochem Syst Ecol 50:322–330. https://doi.org/10.1016/j.bse.2013.05.005

    Article  Google Scholar 

  41. Bousquet J, Strauss S, Doerksen A, Price R (1992) Extensive variation in evolutionary rate of rbcL gene sequences among seed plants. PNAS 89:7844–7848

    Article  Google Scholar 

  42. Berger JD, Buirchell BJ, Luckett DJ, Nelson MN (2012) Domestication bottlenecks limit genetic diversity and constrain adaptation in narrow-leafed lupin (Lupinus angustifolius L.). Theor Appl Genet 124:637–652. https://doi.org/10.1007/s00122-011-1736-z

    Article  Google Scholar 

  43. Teixeira JC, Huber CD (2021) The inflated significance of neutral genetic diversity in conservation genetics. PNAS 118:1–10. https://doi.org/10.1073/pnas.2015096118/-/DCSupplemental

    Article  Google Scholar 

  44. Furlan E, Stoklosa J, Griffiths J et al (2012) Small population size and extremely low levels of genetic diversity in island populations of the platypus, Ornithorhynchus anatinus. Ecol Evol 2:844–857. https://doi.org/10.1002/ece3.195

    Article  Google Scholar 

  45. Ujvari B, Klaassen M, Raven N et al (2018) Genetic diversity, inbreeding and cancer. Proc R Soc B Biol Sci 285:1–8. https://doi.org/10.1098/rspb.2017.2589

    Article  Google Scholar 

  46. Kleinhans C, Willows-Munro S (2019) Low genetic diversity and shallow population structure in the endangered vulture, Gyps coprotheres. Sci Rep 9:1–11. https://doi.org/10.1038/s41598-019-41755-4

    Article  Google Scholar 

  47. Frankham R, Ballou JD, Broscoe DA (2004) A primer of conservation genetics. Cambridge University Press, Cambridge

    Book  Google Scholar 

  48. Guo C, McDowell IC, Nodzenski M et al (2017) Transversions have larger regulatory effects than transitions. BMC Genomics 18:1–9. https://doi.org/10.1186/s12864-017-3785-4

    Article  Google Scholar 

  49. Stoltzfus A, McCandlish DM (2017) Mutational biases influence parallel adaptation. Mol Biol Evol 34:2163–2172. https://doi.org/10.1093/molbev/msx180

    Article  Google Scholar 

  50. Stoltzfus A, Norris RW (2016) On the causes of evolutionary transition: transversion bias. Mol Biol Evol 33:595–602. https://doi.org/10.1093/molbev/msv274

    Article  Google Scholar 

  51. Aesomnuk W, Ruengphayak S, Ruanjaichon V et al (2021) Estimation of the genetic diversity and population structure of Thailand’s rice landraces using SNP markers. Agron 11:995. https://doi.org/10.3390/agronomy11050995

    Article  Google Scholar 

  52. Fernández-García JL (2017) Phylogenetics for wildlife conservation. In: Phylogenetics. InTech, UK

    Google Scholar 

  53. Nyffeler R, Baum DA (2000) Phylogenetic relationships of the durians (Bombacaceae-Durioneae or /Malvaceae/Helicteroideae/Durioneae) based on chloroplast and nuclear ribosomal DNA sequences. Plant Sys Evol 224:55–82

    Article  Google Scholar 

  54. Nyffeler R, Baum DA (2001) Systematics and character evolution in Durio s. lat.(Malvaceae/Helicteroideae/Durioneae or Bombacaceae-Durioneae). Org Divers Evol 1:165–178

    Article  Google Scholar 

  55. Santoso PJ, Saleh GB, Saleh NM, Napis S (2005) Phylogenetic relationships amongst 10 Durio species based on PCR-RFLP analysis of two chloroplast genes. Indon J Agric Sci 6:20–27

    Article  Google Scholar 

  56. Santoso PJ, Indriyani NLP, Istianto M et al (2017) Phylogeny of Indonesian durian (Durio sp.) germplasm based on polymorphism of ITS-nrDNA sequences. Acta Hort 1186:35–41. https://doi.org/10.17660/ActaHortic.2017.1186.5

    Article  Google Scholar 

  57. de Los Reyes BG (2019) Genomic and epigenomic bases of transgressive segregation – New breeding paradigm for novel plant phenotypes. Plant Sci 288:1–10. https://doi.org/10.1016/j.plantsci.2019.110213

    Article  Google Scholar 

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Acknowledgements

We wish to thank Hanif Wicaksono for providing samples of durian leaves for this study. We also thank our colleagues at the Center for the Release and Certification of Seeds for Food Crops and Horticulture, South Kalimantan, Indonesia, for the information provided regarding the existence of these samples.

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Authors and Affiliations

  1. Laboratory of Genetics and Molecular Biology, Faculty of Mathematics and Natural Sciences, University of Lambung Mangkurat, Jl. A. Yani Km. 36, Banjarbaru, South Kalimantan, 70714, Indonesia

    Dindin Hidayatul Mursyidin, Muhammad Irfan Makruf &  Badruzsaufari

  2. Assessment Institute of Agricultural Technology, Jl. Panglima Batur Barat No. 4, Banjarbaru, South Kalimantan, 70714, Indonesia

    Aidi Noor

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  1. Dindin Hidayatul Mursyidin
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  2. Muhammad Irfan Makruf
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  3. Badruzsaufari
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Contributions

DHM conceptualized the overall research, designing and conducting the experiments, and final manuscript preparation. MIR carried out the sample collection, experimentation, data collection, and draft preparation. BZ assisted in partial conceptualization of the research work and contributed to the molecular data analysis. AN contributed in guiding the sample collection. All authors have read and approved the manuscript for publication.

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Correspondence to Dindin Hidayatul Mursyidin.

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Mursyidin, D.H., Makruf, M.I., Badruzsaufari et al. Molecular diversity of exotic durian (Durio spp.) germplasm: a case study of Kalimantan, Indonesia. J Genet Eng Biotechnol 20, 39 (2022). https://doi.org/10.1186/s43141-022-00321-8

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Keywords

  • DNA barcoding
  • Genetic diversity
  • Malvaceae
  • Phylogenetic relationship
  • Plant breeding