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Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus



In fungi, like other eukaryotes, protein turnover is an important cellular process for the controlling of various cellular functions. The ubiquitin-proteasome pathway degrades some selected intracellular proteins and F-box proteins are one of the important components controlling protein degradation. F-box proteins are well studied in different model plants however, their functions in the fungi are not clear yet. This study aimed to identify the genes involved in protein degradation for disease development in the Macrophomina phaseolina fungus.


In this research, in silico studies were done to understand the distribution of F-box proteins in pathogenic fungi including Macrophomina phaseolina fungus. Genome-wide analysis indicates that M. phaseolina fungus contained thirty-one F-box proteins throughout its chromosomes. In addition, there are 17, 37, 16, and 21 F-box proteins have been identified from Puccinia graminis, Colletotrichum graminicola, Ustilago maydis, and Phytophthora infestans, respectively. Analyses revealed that selective fungal genomes contain several additional functional domains along with F-box domain. Sequence alignment showed the substitution of amino acid in several F-box proteins; however, gene duplication was not found among these proteins. Phylogenetic analysis revealed that F-box proteins having similar functional domain was highly diverse form each other showing the possibility of various function. Analysis also found that MPH_00568 and MPH_05531 were closely related to rice blast fungus F-box protein MGG_00768 and MGG_13065, respectively, may play an important role for blast disease development.


This genome-wide analysis of F-box proteins will be useful for characterization of candidate F-box proteins to understand the molecular mechanisms leading to disease development of M. phaseolina in the host plants.


Protein turnover is the balance between protein synthesis and protein degradation. This process is an important regulator of functioning of the different cellular processes including cell cycle, metabolic control, physical development, and various signal transduction pathways [1, 2]. Protein turnover processes are the same in different cells, however highly different in the aspect of turnover control and regulation [3]. Proteins serve a variety of functions within cells and also interact with other proteins, lipids carbohydrates, and even with DNA [4,5,6,7]. Most plants and microorganisms can synthesize proteins inside the cell but animals need to take protein through their daily meal [8]. Protein levels are an important regulator of eukaryotic cell development [9]. Protein synthesis is concluded either by biosynthesis or chemical synthesis procedure.

Proteolysis is the breakdown of protein into smaller polypeptides or amino acids. It is a highly specific process where proteins are hydrolyzed to their specific amino acids [1]. In this process, a diverse group of enzymes and designated proteases is involved. In eukaryotic cells, two major pathways are involved in protein degradation: the lysosomal proteolysis pathway and ubiquitin–proteasome pathway [10]. In the lysosomal proteolysis pathway, cell uptake degraded protein by lysosomes through a non-selective process, but it may become selective during starvation especially under carbon and nitrogen starvation condition [11]. Proteins which are degraded in lysosomal proteolysis pathway are commonly long lived, but their necessities and number are very low. However, most of the intracellular proteins are degraded by another pathway, namely the ubiquitin–proteasome pathway. This pathway is highly specific and targets cytosolic and nuclear proteins for rapid degradation [10, 12].

Ubiquitin is a regulatory protein that is highly conserved in all eukaryotes. Protein degradation under the ubiquitin–proteasome pathway involves three major steps: (i) ATP-dependent activation of ubiquitin by E1 enzyme (ubiquitin-activating enzyme), (ii) transfer of activated ubiquitin to E2 (ubiquitin-conjugating enzyme), and (iii) transfer of ubiquitin to the protein to be degraded by E3 complex (ubiquitin protein ligase) [13]. Polyubiquitinated proteins are recognized by the 26S proteasome in which the targets are specifically degraded. E3 ligases are the F-box protein which form a subunit of SCF complex (Skp, Cullin and F-box containing) and confer specificity for a substrate to be degraded [12]. Studies showed that F-box proteins contain a novel motif, linked to cyclin F along with cell cycle regulators of yeast Cdc4p and Skp2p to Skp1p, which are major components of E3 ligase [14].

The F-box protein was first described as cyclin F in human genome; however, a large number of this protein family exists in different model organisms having various functions [15]. F-box proteins were identified as SCF components; they function as non-SCF complexes, too [16,17,18]. The number of F-box proteins are greatly varying in eukaryotic organisms and found to be comparatively higher in plants due to diverse functions including physical growth and development, floral organogenesis, senescence, and pathogen resistance [19]. In hemibiotrophic fungus Magnaporthe oryzae, F-box protein is crucial for conidiogenesis, fungal growth and development, and finally for virulence [20,21,22]. In addition, F-box proteins were reported to be involved in sexual reproduction, morphogenesis and for disease-causing ability in human pathogen Cryptococcus neoformans, Aspergillus nidulans, and in Candida albicans [1, 23, 24]. The understanding of SCF E3 ligases has largely come from extensive studies in two model yeasts, S. cerevisiae and Schizosaccharomyces pombe. S. cerevisiae has at least 20 proteins containing an F-box domain, and several have been well studied, including glucose repression resistant 1 (Grr1) [15]. Despite extensive studies in both model yeasts, very limited studies of SCF E3 ligases have been reported in other fungi. Recent studies on the function of F-box proteins in pathogenic fungi have revealed that SCF E3 ligases are required for fungal virulence. Because of the proven therapeutic potential of the ubiquitin–proteasome pathway for human diseases [25], it would be very important to understand the molecular basis of how this pathway regulates fungal virulence.

Jute is the second most important natural fiber crop after cotton in Bangladesh and called the golden fiber of Bangladesh because of earning a lot of foreign currency [26]. Recently, raw jute and jute product has been considered as the second foreign earning of Bangladesh [27]. However, jute is affected by several biotic and abiotic stresses throughout its growing season and causing yield loss [28]. Macrophomina phaseolina is one of the most important pathogens of jute plant causing stem rot disease leading to yield loss up to 30% [29]. This pathogen has more than 500 hosts including food crops, pulse crops, jute, cotton, and also other crops. This fungus can solely reduce up to 30% jute yield among the total production loss due to fungal diseases and others [29]. Consider the economic importance of this fungus genomic information has been carried out to understand its high survivability and disease-causing ability.

Based on the importance of F-box protein in eukaryotes, we have systematically performed the bioinformatic analysis to identify the gene structure, sequence alignment, phylogenetic relationship, exon–intron structures, domain of F-box protein in the stem rot fungus M. phaseolina. These results provide an essential understanding of F-box protein in M. phaseolina and constitute a strong foundation for further investigation in regulation of fungal virulence, which may lead to novel approaches in developing new antifungal agents.


Identification of F-box proteins from different fungi

To identify the F-box protein sequences in M. phaseolina, their protein and genome sequence were downloaded from the website of Basic and Applied Research on Jute Project (BARJ) ( and NCBI database ( And another online database, comparative fungal genomic platform (CFGP 2.0) ( was used for the identification of F-box proteins from the selected fungal genomes [30]. In this analysis, a total of 25 fungal genomes (Table 1) were used where Inter Pro domain (IPR001810; IPR006527; IPR007397; IPR012885; IPR013187; IPR017451, and IPR022364) was used as reference for this search. The E-value threshold was selected at 10-3 to get the entire possible F-box protein candidates.

Table 1 Presence of F-box proteins in different economically important fungi and plants

Basic structure and localization

Information of protein length was gathered from the NCBI database ( General feature format (GFF) data were used to identify exon–intron structures of all F-box domain-containing proteins from the M. phaseolina fungus with the help of online software Gene Structure Display Server 2.0 ( Online-based software WoLF PSORT was applied to predict the probable localization of all thirty-one F-box box proteins [32]. Different domains were identified and analyzed with the online software SMART ( and Pfam (

Sequence alignment and chromosomal mapping of F-box proteins

Protein sequences were collected from two different databases ( and, and those sequences were aligned using Clustal Omega software ( The physical locations of Macrophomina phaseolina F-box proteins on respective chromosomes/ scaffolds were identified using BLASTN search against the local Macrophomina phaseolina database as the Macrophomina phaseolina was not assembled at chromosome-scale so assembled sequences in the form of scaffolds were used for chromosomal mapping. The starting position of each protein was shown on the chromosome or scaffolds.

Gene ontology (GO) analysis

Gene ontology (GO) annotation of F-box proteins for understanding the biological processes, cellular components and molecular functions were done through Blast2GO program (

Phylogenetic analysis

The full-length amino acid sequences of putative F-box proteins of stem rot fungus M. phaseolina, and published F-box proteins from different fungal organisms were collected from the CFGP 2.0 ( [30]. In order to understand the relationship among the F-box proteins in M. phaseolina and the selective fungal organism, a phylogenetic tree was constructed by MEGA6 software. Initially, multiple-sequence alignment of abovementioned fungal species of F-box protein sequences were created using the ClustalW tool in MEGA6, and then according to the alignment file, a phylogenetic tree was generated using the neighbor-joining (NJ) method [33] inferred from 1000 bootstrap replicates with other default parameter.


Identification of F-box proteins in different fungi

Members of the F-box protein family consists of a large number of proteins having the F-box domain as a signature. To identify the F-box proteins from different economically important fungi including stem rot fungus Macrophomina phaseolina several InterPro domains were used. In this analysis, the F-box protein from model plants Arabidopsis thaliana, Cicer arietinum, Oryzae sativa, and model fungus rice blast fungus (Magnaporthe oryzae) were also added for the comparison analysis with the F-box proteins from the identified fungal genomes [2, 13, 22, 31]. The genome-wide search found great variations of fungal F-box proteins along with plants where thirty one F-box domain-containing protein was found in jute stem rot fungus M. phaseolina genome (Table 1 and Table S1). Saccharomyces cerevisiae, Schizosaccharomyces pombe, Ustilago maydis, and Puccinia graminis contain 11, 12, 16, and 17, respectively, which were lower in number, whereas Fusarium graminearum, Phanerochaete chrysosporium, and Aspergillus nidulans contain a higher number of F-box protein (Table 1). Domain analysis detected several other functional domains like WD40, LRR, and ankyrin along with F-box domain in the M. phaseolina fungus (Fig. 1). This result might indicate the interacting protein variations in the M. phaseolina fungus.

Fig. 1
figure 1

Schematic diagram of F-box protein with different motifs in the stem rot fungus M. phaseolina

Basic structure and chromosomal distribution of M. phaseolina F-box proteins

Protein structures provide the possible function of that particular protein as well as indicate the origin of that particular gene in a genome. To predict the probable functions of F-box proteins, basic structure was analyzed and summarized in Table 2. Analysis found that > 50% of the identified F-box proteins did not have any intron in their protein structure. In addition, another 23% protein contained two exons and one intron in their protein sequences. It was also found that two proteins namely MPH_10780 and MPH_12786 had eight and twelve exons, respectively; however, these two proteins did not have the highest protein length among the rest of the F-box proteins. MPH_00738 protein showed the highest protein length having only four exons and three introns in the protein structure (Table 2 and Figure S1). Around 55% and 33% F-box proteins were found to be localized in the cytoplasm and nucleus, respectively. However, only two proteins were predicted to localize in the mitochondria and endoplasmic reticulum (ER), respectively. From the localization analysis, it was confirmed that most of the F-box proteins were localized inside the cell, and it will help to design specific antifungal chemicals. Chromosomal locations of the F-box proteins were determined using the draft genome sequences of Macrophomina phaseolina [29]. Thirty [30] F-box proteins were located on nine (09) different chromosomes (Fig. 2). Chromosomal position of MPH_00738 was not found, and chromosomes 8 and 9 were not found to contain any F-box protein. From this result, no cluster of F-box protein was observed, and it indicated that gene duplication event might not have occurred for this protein family in Macrophomina phaseolina.

Table 2 Basic information of F-box protein in the stem rot fungus Macrophomina phaseolina
Fig. 2
figure 2

Chromosomal distribution of Macrophomina phaseolina F-box protein

Sequence alignment of M. phaseolina F-box proteins

To gain the extensive understanding of F-box proteins in the jute stem rot fungus M. phaseolina genome, amino acids of all thirty-one proteins were aligned using Clustal Omega ( Alignment analysis found very low amino acid sequence similarities among the F-box proteins of M. phaseolina except the three amino acids leucine (L), proline (P), and glutamic acid (E) (Fig. 3). However, MPH_09443 proteins did not contain either leucine (L) or proline (P), but solely contained glutamic acid (E), and MPH_12623 proteins had only proline (P), and the other two, leucine (L) and glutamic acid (E), were absent in the F-box domain (Fig. 3). It was also observed that leucine (L) replaced with isoleucine (I), methionine (M), tyrosine (Y), and proline (P) changed with alanine (A), serine (S), and leucine (L) in several proteins. However, glutamic acid (E) only altered with the aspartic acid (D) in three F-box proteins. This might be due to the point mutation in the nucleic acid of these proteins in the M. phaseolina fungus. BLAST results found very low sequence coverage (36% or less) among the F-box proteins with default e-value (Table S2), pointing that the F-box proteins of Macrophomina phaseolina fungus were not duplicated and those are independent proteins.

Fig. 3
figure 3

Sequence alignment of F-box protein in the stem rot fungus M. phaseolina

Classification of M. phaseolina F-box proteins

Based on the presence of different functional domains along with F-box domain, F-box proteins of M. phaseolina fungus were classified. For this analysis, F-box proteins were analyzed using the online software SMART ( and Pfam ( Analysis found that five major subfamilies where nineteen proteins contained only F-box domain and did not have any other functional domain in their C-terminal region (Fig. 4). In addition, five proteins and three proteins contained additional leucine-rich repeats (LRR) and WD40 repeats, respectively along with the both F-box domain. Moreover, ankyrin repeat was found as an additional functional domain with the F-box domain in one protein namely MPH_03805. Three proteins (MPH_00553, MPH_00738, and MPH_12153) contained 3 different additional domains like RNI-like, ZnF-C2H2, and YccV-like along with F-box domain (Table S1). However, no protein was found with an additional domain of unknown function as commonly found in F-box protein of plants genome. This classification might indicate the protein–protein interaction of the additional domain for disease development in M. phaseolina. A gene ontology analysis was also carried out to predict the possible functions of Macrophomina phaseolina F-box proteins. Analysis found that most of the F-box proteins (28 proteins) involved protein binding rather than molecular function, biological process, and cellular component (Table S3).

Fig. 4
figure 4

Classification of F-box proteins in M. phaseolina. The number of F-box proteins were classified and shown in numbers

Phylogenetic analysis of F-box proteins

By using protein sequences, phylogenetic tree was constructed to understand the relationship of F-box proteins in different phytopathogenic fungus. The phylogenetic tree was made through the neighbor-joining approach through MEGA 6.0. It has been found that most of the F-box proteins were highly diverse from one protein to another (Fig. 5). M. phaseolina genome contains five F-box protein namely MPH_04533, MPH_06673, MPH_01929, MPH_06193, and MPH_05531 having leucine-rich repeat (LRR); however, MPH_05531 only showed a close relationship with yeast Grr1, rice blast fungus MoGrr1, and powdery mildew pathogen B. graminis (estExt fgenesh2 pg. C 570056), WD40 repeat containing F-box proteins were mostly found in a similar place in the phylogenic tree; however, WD40 repeat containing F-box proteins of M. phaseolina seemed to diverse from them and found far away from the major subclade of WD40 domain-containing F-box proteins (Fig. 5). Among the four fungal species, M. phaseolina and M. oryzae had one ankyrin repeat-containing F-box protein in each fungus, but they are phylogenetically distant and present in different places in the tree indicating the possibility of interaction with different proteins. From the phylogenetic tree, it can also be predicted that sequence diversity of those proteins may lead to diverge functions in fungi.

Fig. 5
figure 5

Phylogenetic analysis of F-box proteins of Macrophomina phaseolina. Phylogenetic analysis was carried out by MEGA 6.0 program

Comparison of M. phaseolina F-box proteins with other phytopathogenic fungal F-box proteins

It has been reported that protein families vary from organism to organism, and this might help to predict their involvement of physiological process as well as evolution of that particular protein families. In this analysis, F-box proteins of fungi having different lifestyles, and plants were also included to understand the distribution of F-box proteins in different organisms.

Proportion of contained F-box proteins in most selected fungal genomes ranging from 10 to 49% in Phytophthora infestans and Histoplasma capsulatum, respectively (Fig. 6). It is quite interesting that a proportion of F-box proteins in the kingdom Viridiplantae were much higher than the selected fungi used in this study (Table 1). This result might suggest that plants require more F-box proteins for performing various physiological processes to complete their full life cycle, whereas, a lower number of proteins in fungi indicate their importance during the disease development process in the host. Analysis also revealed that presence of F-box proteins had no relationship with the lifestyles (biotrophic, hemibiotrophic, and necrotrophic nature) and also with the host range of selected fungi used in this study. Symbiotic fungus Laccaria bicolor genome contained the highest number of F-box proteins compared with the rest of the fungi; however, the proportions were almost similar with the proportion of other three fungi namely, Mycosphaerella graminicola, Phanerochaete chrysosporium, and Aspergillus fumigatus (Table 1 and Fig. 6).

Fig. 6
figure 6

Comparative analysis of F-box proteins in different economically important fungi

Comparative analysis of sequence alignment found the similar replacement of leucine (L), proline (P), and glutamic (E) are were also observed in the F-box protein of M. oryzae, S. cerevisiae, and B. graminis (Fig. 7). In yeast, S. cerevisiae, leucine (L) and proline (P) were not replaced; however, glutamic acid (E) was replaced by lysine (K), asparagine (N), and leucine (L). In M. oryzae, leucine (L) was replaced by the valine (V), methionine (M), and proline (P) was replaced by alanine (A). In B. graminis, proline (P) was only replaced by the serine (S). However, glutamic acid (E) was replaced by the aspartic acid (D), alanine (A), threonine (T), and serine (S). These results clearly indicate that point mutation is a common event in the living organisms.

Fig. 7
figure 7

Sequence alignment of F-box protein in four different fungi (M. phaseolina, M. oryzae, B. graminis, and S. cerevisiae)

SCF complex of F-box protein (skp2), was highly conserved in most selected biotrophic, hemibiotroph, necrotrophic, and symbiotic fungi (data not shown). This result suggests that phosphorylation process is common in all fungi for their growth and development including disease development. Interestingly, fungi contained comparatively more WD40 repeat than the plant F-box protein suggesting that fungi might require more WD40 for disease development (Table 3). Leucine-rich repeats (LRR) were highly present in plants than the fungi, indicating the importance of LRR for physical growth for survival. Abundance of ankyrin repeat in necrotrophic fungi gives hint that this repeat might be involved in the protein–protein interaction for disease development; however, this function has not been yet reported.

Table 3 Comparison of leucine-rich repeat (LRR), WD40 (WD), and ankyrin (Ank) repeat in fungi


Proteolysis is not only a common process for living organisms but also necessary for protein homeostasis for proper growth and development through the cell division cycle [34]. Several components have been reported to be involved in the proteolysis, and a novel motif called F-box is responsible for the ubiquitin-mediated proteolysis [14].

F-box proteins are highly species-specific, and there might be no relationship between the organism’s genome size with their proportion. Protein number can be changed with the protein loss and gain in the genome [19, 35]. In this research, fungal genome was found to contain different numbers of F-box proteins (Table 1); however, there was no distinct pattern for existing of F-box proteins. The number of F-box proteins in fungal genome was comparatively much lower than the plant genome except symbiotic fungus Laccaria bicolor. Interestingly, the proportion of fungal F-box protein was much smaller than the proportion of plant F-box protein (data not shown). It seemed that fungi need less number of F-box protein for their survival and disease development. This prediction is supported by the research where 24 F-box proteins were identified in rice blast fungus (M. oryzae) genome; however, only three F-box proteins were found to be involved for full virulence [22]. Our identified F-box protein number might be varied because of the parameters (default) that we selected for our BLAST search against the F-box domain. It has been reported that BLAST is sensitive enough to identify the sequences from the remote homologous protein [36].

Exon–intron configuration of the F-box proteins has a distinct feature of having intron-less protein in many plant genomes [37]. Domain arrangement and composition can be resulted through shifting of exon–intron as well as insertion and/ or deletion of exon [19]. Stem rot fungal (M. phaseolina) F-box proteins contained > 50% of the total intron-less protein (Table 2). Although it is not clear how these proteins arisen in the stem rot fungus M. phaseolina genome, it seems to be originated through gene duplication or reverse transcription and integration. It has been reported that the number of genes in eukaryotic organisms can be duplicated through natural selection as well as by reverse transcription and then integration [38, 39]. It was also reported that intron/exon structure of a subfamily had a strong structural relationship between the chickpea F-box proteins [13].

Leucine (L) and proline (P) were reported as the compulsory amino acids for the function of F-box protein in the living organisms [15]. However, leucine (L), proline (P), and glutamic acid (E) were found to be replaced by several other amino acids in different F-box proteins in other four fungi (Fig. 3 and 7). These changes of amino acid might occur due to the single nucleotide substitution during the replication of DNA. It has been reported that single nucleotide replacement for another can occur during the replication of DNA [40, 41]. Amino acid replacement can also create mutational fore in living organisms [42]. It was hypothesized that alteration of amino acid in F-box proteins might lead to diverse functions in fungi. It also reports that due to the amino acid substitution, the similar gene showed diverse function in insulin delivery and reduction of enzymatic activity in human [43, 44].

Protein domains are the small units of evolution as well as the basic components of protein structure and function [45, 46]. In general, fungi contain various numbers of domain compared with the other organisms [47]. Expansion of domain in the fungal genome occurred by domain duplication through recombination [48]. F-box proteins contain generally one or more variable protein–protein interacting domain such as leucine-rich repeat (LRR), kelch repeat, WD40 repeat, and more for interaction with the target protein [49]. Domain analysis revealed that stem rot fungus contains a large fraction (61%) of F-box proteins having only F-box domain (Fig. 4). It is highly likely that many of the F-box proteins might evolve into new ones in the stem rot fungus which had not undergone domain expansion yet or lost the domain for functional losses. In addition, conservation of F-box domain, skp2-like in fungi indicated the dependency of phosphorylation; however, proper–protein degradation relies on the multiple mechanisms [50].

Phylogenetic analysis not only shows the relation among the proteins but also indicate their evolutionary histories including climatic and geographical history on earth [51]. Each fungal F-box proteins were highly divergent in their sequences from others even though they had the similar functional domain (Fig. 5). It was expected that phylogenetically closely located proteins with the similar domain are involved in a similar function to interact with a similar substrate. However, same domain proteins might involve in different functions due to its point mutation in proteins sequences [52]. Similar event in domain shuffling for protein diversification was reported in rice where individual duplication was found [53]. In this paper, genome-wide F-box proteins have been identified in the jute stem rot fungus M. phaseolina that have been believed to be involved in protein degradation.


In this experiment, thirty-one F-box proteins from the jute stem rot fungus M. phaseolina were identified and analyzed. Based on the existence of different domains, all proteins were categorized in five groups. Large group (61%) consists of F-box domain alone; however, leucine-rich repeat (LRR) and WD40-containing group were in the second and third largest group, respectively. Single nucleotide substitution resulted in leucine (L), proline (P), and glutamic acid (E) in several F-box proteins in different fungi. Phylogenetic tree revealed that proteins from the same group are highly diverse from each other indicating the diverse functions of F-box proteins in fungi. These results provide an essential understanding of F-box protein in M. phaseolina and constitute a strong foundation for further investigation in the regulation of fungal virulence, which may lead to novel approaches in developing new antifungal agents.

Availability of data and materials

All the protein sequences are available in the NCBI database.



Deoxyribonucleic acid


Adenosine triphosphate


Skp, Cullin and F-box containing


Glucose repression resistant 1


Basic and Applied Research on Jute Project


National Center for Biotechnology Information


Comparative fungal genomic platform


General feature format


Molecular Evolutionary Genetics Analysis




Leucine-rich repeat


Endoplasmic reticulum


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Authors are thankful to Basic and Applied Research on Jute Project, Bangladesh Jute Research Institute for pursuing research activities.


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Authors and Affiliations



MAS, MWU, and MSI designed the study. MAS, KKB, QMMH, and MZT performed the experiments and analyzed the data. MAS wrote the manuscript. MUW, KKB, and MSI edited and finalized the manuscript and supervised the overall study. The authors read and approved the final manuscript.

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Correspondence to Md. Abu Sadat.

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Supplementary Information

Additional file 1: Table S1.

Identified F-box proteins in the stem rot fungus Macrophomina phaseolina.

Additional file 2: Table S2.

BLAST search result of F-box protein of Macrophomina phaseolina.

Additional file 3: Table S3.

BLAST2GO analysis of Macrophomina phaseolina F-box proteins.

Additional file 4: Figure S1.

Exon-intron structure and length F-box protein in stem rot fungus M. phaseolina.

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Sadat, M.A., Ullah, M.W., Bashar, K.K. et al. Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus. J Genet Eng Biotechnol 19, 46 (2021).

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