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Table 1 Effect of cytokinins and NAA on shoot development from shoot tip explants of S. mollis inoculated onto MS medium after 8 weeks of culture

From: In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

Plant growth regulators (μM) Explants with shoots (%) No. of shoots per explant (#) Shoot length (cm) (#)
MS0    
BAP    
 2.2 68.24 10.74 ± 0.5g 2.5 ± 0.6d
 4.4 75.81 19.22 ± 0.78c 3.5 ± 0.5bc
 8.9 96.27 25.32 ± 0.83a 4.5 ± 0.8a
 11.1 66.58 20.21 ± 0.8b 4.2 ± 0.6ab
TDZ    
 2.22 54.59 13.22 ± 0.5f 1.5 ± 0.69e
 4.54 78.69 21.54 ± 0.3b 3.5 ± 0.4bc
 6.81 65.38 17.21 ± 0.29cd 2.7 ± 0.8d
Kinetin    
 2.32 53.87 11.15 ± 0.3g 2.5 ± 0.78d
 4.6 68.18 18.69 ± 0.28cd 3.5 ± 0.42bc
 6.9 76.78 19.86 ± 0.36c 4.3 ± 0.2a
 9.3 70.09 17.85 ± 0.3cd 4.1 ± 0.39ab
BAP + NAA    
 8.9 + 0.53 85.98 25.26 ± 0.39a 4.1 ± 0.68ab
 8.9 +1.59 82.21 22.68 ± 1.3b 3.9 ± 0.7b
 8.9 + 2.65 80.23 18.26 ± 0.4cd 3.9 ± 0.6b
TDZ+NAA    
 4.5 + 0.53 78.65 20.21 ± 0.31c 2.5 ± 0.46d
 4.5 +1.59 72.34 18.96 ± 0.42cd 2.4 ± 0.4d
 4.5 + 2.65 69.23 16.17 ± 0.47de 2.4 ± 0.41d
Kinetin + NAA    
 6.9 + 0.53 76.16 19.21 ± 0.71c 4.0 ± 0.8ab
 6.9 +1.59 74.25 17.11 ± 0.29cd 3.9 ± 0.7b
 6.9 + 2.65 68.95 13.24 ± 0.2f 3.9 ± 0.9b
  1. #Data are presented as the mean ±SD (n=20)
  2. Means followed by different letter within columns indicate significant differences at p≤0.05