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Table 1 Effect of cytokinins and NAA on shoot development from shoot tip explants of S. mollis inoculated onto MS medium after 8 weeks of culture

From: In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

Plant growth regulators (μM)

Explants with shoots (%)

No. of shoots per explant (#)

Shoot length (cm) (#)

MS0

   

BAP

   

 2.2

68.24

10.74 ± 0.5g

2.5 ± 0.6d

 4.4

75.81

19.22 ± 0.78c

3.5 ± 0.5bc

 8.9

96.27

25.32 ± 0.83a

4.5 ± 0.8a

 11.1

66.58

20.21 ± 0.8b

4.2 ± 0.6ab

TDZ

   

 2.22

54.59

13.22 ± 0.5f

1.5 ± 0.69e

 4.54

78.69

21.54 ± 0.3b

3.5 ± 0.4bc

 6.81

65.38

17.21 ± 0.29cd

2.7 ± 0.8d

Kinetin

   

 2.32

53.87

11.15 ± 0.3g

2.5 ± 0.78d

 4.6

68.18

18.69 ± 0.28cd

3.5 ± 0.42bc

 6.9

76.78

19.86 ± 0.36c

4.3 ± 0.2a

 9.3

70.09

17.85 ± 0.3cd

4.1 ± 0.39ab

BAP + NAA

   

 8.9 + 0.53

85.98

25.26 ± 0.39a

4.1 ± 0.68ab

 8.9 +1.59

82.21

22.68 ± 1.3b

3.9 ± 0.7b

 8.9 + 2.65

80.23

18.26 ± 0.4cd

3.9 ± 0.6b

TDZ+NAA

   

 4.5 + 0.53

78.65

20.21 ± 0.31c

2.5 ± 0.46d

 4.5 +1.59

72.34

18.96 ± 0.42cd

2.4 ± 0.4d

 4.5 + 2.65

69.23

16.17 ± 0.47de

2.4 ± 0.41d

Kinetin + NAA

   

 6.9 + 0.53

76.16

19.21 ± 0.71c

4.0 ± 0.8ab

 6.9 +1.59

74.25

17.11 ± 0.29cd

3.9 ± 0.7b

 6.9 + 2.65

68.95

13.24 ± 0.2f

3.9 ± 0.9b

  1. #Data are presented as the mean ±SD (n=20)
  2. Means followed by different letter within columns indicate significant differences at p≤0.05