Table 3 Polymorphism of 24 TRAP marker combinations on 30 sweet sorghum genotypes
Gene function | Primer (marker) combinations | Total number of bands | Number of polymorphic bands | Polymorphic bands (%) | |
|---|---|---|---|---|---|
Forward (fixed) | Reverse (arbitrary) | ||||
Lignin biosynthesis | Cinnamoyl coA reductase (CCR) | Em01 | 54 | 36 | 66.67 |
Em02 | 63 | 36 | 57.14 | ||
Em03 | 63 | 45 | 71.43 | ||
Em04 | 72 | 63 | 87.50 | ||
Cinnamoyl alcohol dehydrogenase (CAD) | Em01 | 90 | 63 | 70.00 | |
Em02 | 81 | 45 | 55.56 | ||
Em03 | 63 | 36 | 57.14 | ||
Em04 | 63 | 36 | 57.14 | ||
Caffeic acid 3-O-methyltransferase (COMT) | Em01 | 108 | 90 | 83.33 | |
Em02 | 99 | 81 | 81.82 | ||
Em03 | 81 | 63 | 77.78 | ||
Em04 | 90 | 72 | 80.00 | ||
Total | 927 | 666 | Â | ||
Mean | 142.62 | 102.46 | 70.46 | ||
Sucrose biosynthesis | Sucrose synthase (SUSy) | Em01 | 45 | 36 | 80.00 |
Em02 | 54 | 36 | 66.67 | ||
Em03 | 36 | 27 | 75.00 | ||
Em04 | 45 | 27 | 60.00 | ||
Sucrose phosphate synthase (SPS) | Em01 | 72 | 54 | 75.00 | |
Em02 | 90 | 54 | 60.00 | ||
Em03 | 45 | 27 | 60.00 | ||
Em04 | 63 | 45 | 71.43 | ||
Soluble acid invertase (Inv) | Em01 | 72 | 54 | 75.00 | |
Em02 | 54 | 36 | 66.67 | ||
Em03 | 81 | 63 | 77.78 | ||
Em04 | 54 | 36 | 66.67 | ||
Total | 711 | 495 | Â | ||
Mean | 59.25 | 41.25 | 69.52 | ||
Grand total | 1638 | 1161 | Â | ||
Grand mean | 68.25 | 48.38 | 70.88 | ||