Table 2 Fixed and arbitrary primers used as TRAP markers
|  | Gene/primer name | Gene ID/accession | Nucleotide sequence (5′ > 3′) | Tm |
|---|---|---|---|---|
Lignin-related genes | Cinnamoyl coA reductase (CCR) | LOC8054741 | GTCAGGAACCCAGATGAC | 55 |
Cinnamoyl alcohol dehydrogenase (CAD) | LOC110434683 | GGGCTTCAAAGTACCCTA | 54 | |
Caffeic acid 3-O-methyltransferase (COMT) | LOC8070884 | CAAGAAGCTCCTCGAGTT | 54 | |
Sucrose-related genes | Sucrose synthase (susy) | Sb01g033060 | ATGGTATTCTCCGCAAGTGG | 58 |
Soluble acid invertase (Inv) | Sb04g000620 | CATCGTTGCAGGGTATCCC | 59 | |
Sucrose phosphate synthase (sps) | Sb05g007310 | GCAAACCTTACGCTGATACTG | 56 | |
Arbitrary primer | Em1 | Â | GACTGCGTACGAATTTGC | 49 |
Em2 | Â | GACTGCGTACGAATTGAC | 53 | |
Em3 | Â | GACTGCGTACGAATTTGA | 53 | |
Em4 | Â | GACTGCGTACGAATTAAT | 52 |