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Journal of Genetic Engineering and Biotechnology
Fig. 4 | Journal of Genetic Engineering and Biotechnology

Fig. 4

From: Saccharomyces cerevisiae ER membrane protein complex subunit 4 (EMC4) plays a crucial role in eIF2B-mediated translation regulation and survival under stress conditions

Fig. 4

Spot assay to test the effect of H2O2, ethanol, and caffeine on the growth of eIF2B mutants. Hydrogen peroxide, ethanol, and caffeine spot assays were performed with gcd7-201 gcn2∆, GCD7 gcn2∆, and GCD7 GCN2. Ten-fold serially diluted transformants harboring empty hc, vector pEG (KG), or hc/EMC4 were spotted on SC-Ura or SC-Ura/3-AT agar plates supplemented with (a) H2O2 (4 mM), (b) ethanol (10%), and (c) caffeine (30 mM) in the presence of either raffinose or galactose. Cultures were incubated for 2 days at 30 °C

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