Fig. 6

The cell morphology of the strains carrying the swi1-V5 allele across 24 h after thiamine induction. The figure shows the cells of the (a) swi1-V5 strain SPSC 1096, (b) swi1Δ swi1-V5 strain SPSC 1098 and cswi1Δ swi1-V5 rad4-116 strain SPSC 1099 at × 100 magnification stained with DAPI to show the nuclei at the 0 h time point and the 24 h timepoint. An additional time point at 12 h is shown for the SPSC 1099 strain. For SPSC 1096 and SPSC 1098, there was no significant difference between the cells across those 2 time points in terms of cell length, cell shape and nuclear shape. For SPSC 1099, the cells showed a distinct phenotype, that developed from timepoint 0 to 12 to 24 h, for cells to increase in length. The nuclei of most of these cells showed a fragmentation that became more pronounced at the 24-h timepoint and some cells showed a compacted nucleus. Some mutant cells showed an abnormal cell shape with one nucleus and three ends to the cytoplasm. The phenotypical effect of thiamine addition on the swi1-V5 strains across 24 h