Table 2 List of PCR primer pairs used for amplification
From: Construction of a shuttle expression vector for lactic acid bacteria
Primer pair, forward/reverse | Primer sequence (5′-3′)a | Template DNA | Target gene | Amplicon length (bp) |
|---|---|---|---|---|
pedF pedR | BamHI-GGATCCTATCTAACTAATACTTGACA SphI-GCATGCCGCGAGGATTTCAC | Plasmid pCP289 | pedA-D | 3541 |
oriF oriR | SacI-GAGCTCATGGTAGCCTCTG KpnI-GGTACCGCTTACATTAACTT | Plasmid pCP289 | repB,ori | 1557 |
gfpF gfpR | KpnI-GGTACCAGTGTGTTGATAGTGCAGTAT BamHI-GGATCCTTATTTATATAATTCATCCATAC | Plasmid pMK-RQ | gfp | 867 |