Skip to main content

Table 2 List of PCR primer pairs used for amplification

From: Construction of a shuttle expression vector for lactic acid bacteria

Primer pair, forward/reversePrimer sequence (5′-3′)aTemplate DNATarget geneAmplicon length (bp)
pedF
pedR
BamHI-GGATCCTATCTAACTAATACTTGACA
SphI-GCATGCCGCGAGGATTTCAC
Plasmid pCP289pedA-D3541
oriF
oriR
SacI-GAGCTCATGGTAGCCTCTG
KpnI-GGTACCGCTTACATTAACTT
Plasmid pCP289repB,ori1557
gfpF
gfpR
KpnI-GGTACCAGTGTGTTGATAGTGCAGTAT
BamHI-GGATCCTTATTTATATAATTCATCCATAC
Plasmid pMK-RQgfp867
  1. aRestriction enzyme sites are underlined
\