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Table 1 Physicochemical properties of the HPIV-3 proteins analyzed through the ProtParam tool

From: Immunoinformatics-aided rational design of multiepitope-based peptide vaccine (MEBV) targeting human parainfluenza virus 3 (HPIV-3) stable proteins

Proteins

Molecular weight

Theoretical pI

Instability index

Stability profiling

Aliphatic index

Grand average of hydropathicity

Nucleoprotein (515 aa)

57,786.07

5.21

45.31

Unstable

83.38

 − 0.428

Phosphoprotein (602 aa)

67,433.36

5.55

47.56

Unstable

63.95

 − 1.051

D protein (373 aa)

41,398.69

5.30

57.75

Unstable

46.54

 − 1.323

C protein (199)

23,297.94

9.71

56.14

Unstable

92.56

 − 0.700

Fusion glycoprotein F0

60,034.26

6.42

40.87

Unstable

109.94

 − 0.003

Matrix protein (353 aa)

39,530.30

9.59

38.75

Stable

100.74

 − 0.070

Hemagglutinin-neuraminidase

64,159.07

8.09

30.61

Stable

92.48

 − 0.263

RNA-directed RNA polymerase L (2233)

255,944.92

6.12

36.99

Stable

98.28

 − 0.243