Application of statistical methodology for the optimization of l-glutaminase enzyme production from Streptomyces pseudogriseolus ZHG20 under solid-state fermentation

Journal of Genetic Engineering and Biotechnology

Table 2 Central composite design table

Run	Wheat bran	Sesame oil seed cake	Corn steep liquor	Predicted	l-glutaminase activity U/gds
1	3.75	2	0.4	753.21	877.02
2	2.5	1	0.2	508.26	329.51
3	2.5	3	0.2	775.95	879.73
4	3.75	2	0.4	753.21	856.16
5	5	3	0.2	1211.69	1299.36
6	3.75	3.68	0.4	779.14	819.37
7	5	3	0.2	1211.69	998.01
8	3.75	2	0.4	753.21	881.81
9	2.5	1	0.2	508.26	564.73
10	5.85	2	0.4	968.55	1247.98
11	2.5	1	0.6	317.97	471.8
12	2.5	3	0.2	775.95	862.58
13	1.64	2	0.4	366.55	319.22
14	2.5	3	0.6	159.22	79.88
15	2.5	3	0.6	159.22	332.83
16	5	3	0.6	762.2	845.41
17	3.75	2	0.06	884.78	931.95
18	5.85	2	0.4	968.55	838.9
19	3.75	2	0.4	753.21	292.89
20	5	1	0.2	621.17	639.051
21	3.75	2	0.4	753.21	856.94
22	2.5	1	0.6	317.97	629.06
23	3.75	2	0.4	753.21	855.22
24	5	1	0.2	621.17	435.29
25	5	3	0.6	762.2	945.47
26	3.75	2	0.4	753.21	733.91
27	3.75	3.68	0.4	779.14	636.21
28	3.75	2	0.4	753.21	868.68
29	3.75	2	0.4	753.21	880.53
30	3.75	2	0.4	753.21	998.03
31	3.75	0.31	0.4	416.08	162.17
32	2.5	3	0.2	775.95	704.78
33	5	1	0.6	598.14	765.82
34	5	3	0.2	1211.69	1245.62
35	3.75	2	0.4	753.21	857.87
36	3.75	2	0.4	753.21	856.41
37	3.75	2	0.73	346.8	78.14
38	3.75	3.68	0.4	779.14	687.67
39	3.75	0.31	0.4	416.08	734.56
40	3.75	2	0.4	753.21	695.48
41	3.75	2	0.4	753.21	741.4
42	3.75	2	0.4	753.21	794.26
43	3.75	2	0.73	346.8	261.43
44	5	1	0.2	621.17	638.89
45	5.85	2	0.4	968.55	945.11
46	3.75	2	0.4	753.21	745.98
47	2.5	3	0.6	159.22	543.6
48	5	3	0.6	762.2	582.72
49	3.75	2	0.4	753.21	606.61
50	3.75	2	0.06	884.78	825.92
51	5	1	0.6	598.14	551.78
52	3.75	2	0.4	753.21	238.31
53	3.75	0.31	0.4	416.08	81.32
54	5	1	0.6	598.14	685.95
55	2.5	1	0.6	317.97	273.51
56	1.64	2	0.4	366.55	105.95
57	2.5	1	0.2	508.26	871.91
58	3.75	2	0.73	346.8	148.47
59	1.64	2	0.4	366.55	83.76
60	3.75	2	0.063	884.78	984.49