Table 1 Test information and results
Test | Purpose of the test | Validation of the result | |
|---|---|---|---|
Positive | Negative | ||
Indole test | Screen for the organism that degrades tryptophan and produces indole | Cherry red ring formation | Yellow/no ring formation |
Methyl red | Detects if the organism performs mixed acid fermentation | Red | Yellow |
Voges Proskauer | Detects the presence of acetoin within the metabolic pathway | Red/purple color | No color |
Citrate utilization | Screen for the organism that uses citrate as carbon and energy source | Blue | Green |
Catalase | Used for the detection of enzyme catalase | Effervescence | No effervescence |
Coagulase | Used for the detection of protein coagulase | Clot formation | No clot |
Oxidase | Assay for the presence of cytochrome oxidase | Purple | Yellow/no color |
Urease | To test the ability of microbe that degrades urea | Pink | Yellow |
Triple sugar iron agar | Determination of carbohydrate fermentation and H2S production | Pink with gas | Yellow |
Blood hemolysis | Test for the ability of organism that produces haemolysin | Growth seen | No growth |
Starch hydrolysis | Test for the ability of organism that hydrolyses starch | Blue with zone of inhibition | No zone |
Organic acid production | Detection of microbe if it produces any organic acid as an intermediate | Thick white colony growth | Pale /transparent growth |