Cloning and characterization of an acidic lipase from a lipolytic bacterium in tempeh

Journal of Genetic Engineering and Biotechnology

Table 2 Optimization of host and vector systems in the expression of lipase EMP48-D

Host	Vector	Clear zone index^a	Pellet activity (U/mg)
Wild-type	n/a	1.25	17.02 ± 3.01
E. coli DH5α	pGEM-T Easy	n/a	2.11 ± 1.09
E. coli DH5α	pUC-19	n/a	0.93 ± 0.07
*E. coli* BL21 (DE3)	pLysS + pET15-b	1.0	15.02 ± 2.33
*E. coli* BL21 (DE3)	pET15-b	0.95	14.47 ± 1.99
E. coli BL21 with Origami variants	pG-KJE8 + pET15-b	0.75	10.33 ± 2.88
	pGro7 + pET15-b	0.45	7.65 ± 1.56
	pKJE7 + pET15-b	0.7	10.02 ± 0.98
	pG-Tf2 + pET15-b	0.65	8.57 ± 2.55
	pTf16 + pET15-b	0.4	5.35 ± 1.62

^aThe clear zone index was measured after 24 h of incubation on tributyrin agar and was calculated as the ratio of the diameter of the clear zones to the diameter of colonies
The activity was expressed as the mean ± standard deviation