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Journal of Genetic Engineering and Biotechnology
Fig. 4 | Journal of Genetic Engineering and Biotechnology

Fig. 4

From: A scalable overexpression of a thermostable recombinant poly-histidine tag carboxyl esterase under lambda promoter: purification, characterization, and protein modelling

Fig. 4

SDS-PAGE analysis of expression of EstB-polyhistidine. A Protein detection by staining the gel with Coomassie brilliant blue. B In situ activity detection by staining the gel with α-naphthyl acetate and Fast Red TR. Lane 1: crude cell extract of E. coli DH5α/pCYTEX-6X his EstB after induction; lanes 2, 3, and 4: the most active purified fraction of EstB-polyhistidine developed after IMAC and gel filtration, respectively

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