Table 2 Primers used for the amplification of HMRGs
Heavy metal | Genes | Primer sequences | Size | Annealing temperature (0C) |
|---|---|---|---|---|
Cd | cadD | AATTGCAAGTTGTGGTGCAG CCCACACCAGGAATTCTAGC | 155 | 57 |
Co, Ni | cnrA | CCTACGATCTCGCAGGTGAC GCAGTGTCACGGAAACAACC | 422 | 57 |
Cu | pcoC | TTCTTACAGGTGGCCTCGTT CCGGTAATAGGGTGCGTATC | 333 | 57 |
Pb | pbrT | AGCGCGCCCAGGAGCGCAGCGTCTT GGC TCG AAG CCG TCG AGR TA | 448 | 57 |
Hg | merA | GAGATCTAAAGCACGCTAAGGC GGAATCTTGACTGTGATCGGG | 1011 | 57 |