Table 3 Comparison of crude and purified Pfu pol in a 50-mL culture. The values are mean of triplicate samples ± standard deviation
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Crude | 45,260 ± 1530 | 20.09 ± 1.68 | 2253 | 1 | 100 |
HisTrap™ HP | 21,067 ± 1030 | 5.02 ± 0.25 | 4197 | 1.86 | 46 |
HiTrap™ Q HP | 19,620 ± 880 | 1.34 ± 0.08 | 14,641 | 6.5 | 43 |