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Table 2 Comparison of wild-type and codon-optimized sequences using several parameters

From: Codon optimization of a gene encoding DNA polymerase from Pyrococcus furiosus and its expression in Escherichia coli

Sequences

CAI

GC content (%)

Nc

Rare codon (%)

Negative CIS elements

Wild-type

0.53

39.3

47.4

17

5

Optimized

0.8

49.5

39.9

1

1