Codon optimization of a gene encoding DNA polymerase from Pyrococcus furiosus and its expression in Escherichia coli

Journal of Genetic Engineering and Biotechnology

Table 1 Codon distribution of the wild-type versus the codon-optimized sequence

Amino acid	Codon	Host fraction	Pfu DNA polymerase gene
Amino acid	Codon	Host fraction	Wild-type	Optimized
F	UUU	28.9	14	14
F	UUC	18.8	13	13
L	UUA	17.5	11	2
L	UUG	18.6	7	9
L	CUU	12.7	18	3
L	CUC	14.1	17	1
L	CUA	3.4	13	0
L	CUG	54.9	1	52
I	AUU	33.9	31	31
I	AUC	31.0	7	40
I	AUA	5.0	33	0
M	AUG	37.4	10	10
V	GUU	19.6	23	20
V	GUC	14.3	4	15
V	GUA	10.6	19	1
V	GUG	33.9	6	16
S	UCU	8.5	2	3
S	UCC	8.0	2	4
S	UCA	6.1	7	0
S	UCG	11.4	2	2
P	CCU	5.8	9	2
P	CCC	2.4	6	2
P	CCA	7.4	21	4
P	CCG	24.9	0	30
T	ACU	7.7	14	3
T	ACC	25.2	4	17
T	ACA	6.1	10	0
T	ACG	14.6	3	11
A	GCU	13.8	13	5
A	GCC	25.5	8	8
A	GCA	19.6	19	10
A	GCG	32.6	4	21
Y	UAU	18.6	24	20
Y	UAC	8.5	22	26
*	UAA	1.9	0	1
*	UAG	0.3	1	0
H	CAU	9.3	8	8
H	CAC	7.2	6	12
Q	CAA	13.5	11	7
Q	CAG	24.7	4	8
N	AAU	21.2	9	9
N	AAC	15.9	10	10
K	AAA	29.2	37	43
K	AAG	8.8	45	39
D	GAU	30.0	26	17
D	GAC	15.1	12	21
E	GAA	29.4	50	48
E	GAG	18.0	38	40
C	UGU	4.2	2	2
C	UGC	5.8	2	2
*	UGA	0.8	0	0
W	UGG	12.7	11	11
R	CGU	16.4	1	29
R	CGC	18.8	0	15
R	CGA	2.4	0	0
R	CGG	5.0	0	0
S	AGU	9.0	6	0
S	AGC	14.3	6	16
R	AGA	2.4	28	1
R	AGG	2.1	16	0
G	GGU	24.4	6	32
G	GGC	33.1	8	18
G	GGA	8.2	29	0
G	GGG	14.3	7	0