Fig. 5From: Codon optimization of a gene encoding DNA polymerase from Pyrococcus furiosus and its expression in Escherichia colia SDS-PAGE analysis. Lane M: protein marker; lane 1: crude protein, lane 2: Pfu pol purified using HisTrap™ HP column; and lane 3: Pfu pol purified using HiTrap™ Q HP column. b Western blot analysis. Lane M: protein marker and lane 1: purified Pfu pol. The triangle symbol shows the targeted protein bandBack to article page