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Journal of Genetic Engineering and Biotechnology
Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Codon optimization of a gene encoding DNA polymerase from Pyrococcus furiosus and its expression in Escherichia coli

Fig. 3

Expression of Pfu pol in non-transformant and transformant strains of E. coli BL21. The triangle symbol marks the targeted protein band. Lane M: protein marker; lanes 1 and 4: total fraction; lanes 2 and 5: soluble fraction; lanes 3 and 6: insoluble fraction; lanes 1, 2, and 3: non-transformant strain showing no Pfu pol expression; and lanes 4, 5, and 6: transformant strain showing overexpressed Pfu pol

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