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Journal of Genetic Engineering and Biotechnology
Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: Codon optimization of a gene encoding DNA polymerase from Pyrococcus furiosus and its expression in Escherichia coli

Fig. 2

Expression of recombinant protein of Pfu pol in E. coli BL21 transformants with IPTG induction. The triangle symbol indicates the targeted protein band. Lane M: protein marker; lanes 1, 4, and 7: total fraction; lanes 2, 5, and 8: soluble fraction; lanes 3, 6, and 9: insoluble fraction; lanes 1, 2, and 3: transformant colony number 01 (TC-01); lanes 4, 5, and 6: transformant colony number 02 (TC-02); and lanes 7, 8, and 9: transformant colony number 03 (TC-03)

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