Table 1 Specific primers are used to detect FolE and RecA genes in three LAB strains and standard one
Primers | Sequence (5'- > 3') | Template strand | Length | Tm | GC% |
|---|---|---|---|---|---|
FW 1FolE | GCCAGCATAGTCTGAAGTGT | Plus | 20 | 57.61 | 50.00 |
RW 1 FolE | GCAAAAAGCCAACGATGCAAG | Minus | 21 | 59.81 | 47.62 |
FW 2 FolE | TCAACTAGCCGCGGAATTTT | Plus | 20 | 58.18 | 45.00 |
RW 2 FolE | TGGCAGTAGGTGAAGATCCTG | Minus | 21 | 59.17 | 52.38 |
FW 1RecA | GCCCTAATTGGTCCAGGCG | Plus | 19 | 44.3 | 44.3 |
RW 1 RecA | ACAACGGCGTTCTCTCCTAT | Minus | 20 | 44.3 | 44.4 |
FW 2 RecA | ACACAACGTCATTGCAAATGTGA | Plus | 23 | 44.3 | 44.3 |
RW 2 RecA | GCCTGGACCAATTAGGGCAT | Minus | 20 | 44.3 | 44.3 |