Acetaminophen-traces bioremediation with novel phenotypically and genotypically characterized 2 Streptomyces strains using chemo-informatics, in vivo, and in vitro experiments for cytotoxicity and biological activity

Journal of Genetic Engineering and Biotechnology

Table 8 IC50 µg/ml identification of acetaminophen/paracetamol bio-degradation products in vitro cytotoxicity MTT assay using HepG2 and MCF7 cancer cell lines

The selected bacterial isolates M33 and RS2 strains, from samples 1a, 1b, 2a, and 2b in vitro cytotoxicity MTT assay against acetaminophen/paracetamol (positive control) using 2 different cancer cell lines (HepG2 and MCF7) as well as a control one
SH, shaking state; ST, static state; APAP, acetaminophen/paracetamol
DMSO is the negative control. % death rate = 100 – (% cell viability), % cell viability = (mean absorbance of treated sample/mean absorbance of negative control sample) × 100, and the inhibitory concentration of 50% (IC50 µg/ml) measured from the exponential curve of viability against concentration (dose–response curve) using Master –plex-2010 program. All experiment conditions were done in triplicates