Fig. 2
From: Recent advances in genome annotation and synthetic biology for the development of microbial chassis
Illustration of the two different genome minimization strategies. A The top-down genome minimization approach. DELEAT-v0.1 and MinGenome are examples of tools to design minimal genomes using the top-down strategy. B The bottom-up genome minimization approach, where well-characterized, reliable, and context-independent biological parts are constructed into a minimal genome