Table 4 The matrix of the experimental values and response values of BMDC marker expression conditions using (22) factorial design
Run | Factor 1 (A) | Factor 2 (B) | Response 1 | Response 2 | Response 3 | Response 4 |
|---|---|---|---|---|---|---|
Cytokine combinations (ng/mL) | Cell density (cells/mL) | CD14 expression (MFI AU) | CD83 expression (MFI AU) | CD80 expression (MFI AU) | CD86 expression (MFI AU) | |
1 | GM-CSF | 2 × 106 | 272.53 | 75.63 | 133.14 | 186.48 |
2 | GM-CSF | 2 × 106 | 290.32 | 90.32 | 130.2 | 189.35 |
3 | GM-CSF | 2 × 106 | 285.89 | 62.32 | 132.18 | 183.65 |
4 | GM-CSF + IL4 | 2 × 106 | 170.2 | 159.05 | 315.52 | 395.62 |
5 | GM-CSF + IL4 | 2 × 106 | 158.97 | 150.32 | 320.02 | 400.7 |
6 | GM-CSF + IL4 | 2 × 106 | 189.32 | 163.52 | 312.96 | 375.5 |
7 | GM-CSF | 4 × 106 | 194.23 | 105.75 | 137.89 | 214.01 |
8 | GM-CSF | 4 × 106 | 215.32 | 100.35 | 133.85 | 210.36 |
9 | GM-CSF | 4 × 106 | 178.24 | 110.25 | 135.23 | 220.32 |
10 | GM-CSF + IL4 | 4 × 106 | 168.17 | 134.82 | 230.95 | 265.31 |
11 | GM-CSF + IL4 | 4 × 106 | 150.52 | 130.25 | 228.52 | 275.2 |
12 | GM-CSF + IL4 | 4 × 106 | 189.52 | 139.96 | 233.12 | 257.98 |