Fig. 2
Morphological changes of mature BMDCs. A, B Unstimulated DCs (controls) were cultured for 7 days without cytokines at cell densities of 4 × 106 and 2 × 106 cells/mL, respectively, and appeared rounded in shape with no cytoplasmic projections. C DCs were cultured in the presence of GM-CSF and IL4 and seeded with a density of 2 × 106 cells/mL. D DCs were cultured in the presence of GM-CSF and IL4 and seeded with a density of 4 × 106 cells/mL while in E DCs were cultured in the lack of IL4 and with a density of 2 × 106 cells/mL (the weakest cell appearance among other combination) and lastly in F DCs were also supplemented with only GM-CSF and lack of IL4 with a density of 4 × 106 cells/mL. Pictures were taken with an inverted microscope (magnifications were 40 ×); arrows indicate dendrons of DCs