Table 9 The results of the primer set specificity test T1_6 with the BLAST method
Primer | Query cover (%) | Identity (%) | Mismatch (bp) | Potential hybridization | Accession | |
|---|---|---|---|---|---|---|
End 3′ | Total | |||||
T1_6—F3 (21 bp) | 100 | 100 | 0 | 0 | Bat SARS CoV Longquan | KF294457.1 |
100 | 95,2 | 0 | 1 | Bat SARS CoV ZC45 | MG772933.1 | |
85 | 100 | 1 | 3 | HCoV NL63 | DQ445911.1 | |
71 | 93.3 | 1 | 7* | MERS CoV | ON325308.1 | |
71 | 93,3 | 5* | 7* | Influenza A | KX977705.1 | |
52 | 100 | 5* | 10* | HCoV OC43 | MK303624.1 | |
52 | 100 | 3* | 10* | Human rubulavirus 2 | MG836425.1 | |
T1_6—B3 (21 bp) | 100 | 95.2 | 0 | 1 | Bat SARS CoV Longquan | KF294457.1 |
100 | 95,2 | 0 | 1 | Bat SARS CoV ZC45 | MG772933.1 | |
76 | 93,8 | 0 | 6 | Influenza A | KM027559.1 | |
66 | 100 | 1 | 7* | Human metapneumovirus | KJ627417.1 | |
61 | 100 | 3* | 8* | HCoV NL63 | MN306040.1 | |
57 | 100 | 2 | 9* | MERS CoV | MN481983.1 | |
T1_6—F2 (24 bp) | 100 | 100 | 0 | 0 | Bat SARS CoV ZC45 | MG772933.1 |
70 | 94.1 | 2 | 8* | Influenza A | GU396969.1 | |
T1_6—F1c (24 bp) | 95 | 100 | 0 | 1 | Bat SARS CoV Longquan | KF294457.1 |
95 | 100 | 0 | 1 | Bat SARS CoV ZC45 | MG772933.1 | |
66 | 9 3.8 | 1 | 9* | Influenza A | MW872913.1 | |
50 | 100 | 0 | 12* | HCoV OC43 | MN310476.1 | |
50 | 100 | 2 | 12* | Human metapneumovirus | AY525843.1 | |
T1_6—B2 (22 bp) | 100 | 100 | 0 | 0 | Bat SARS CoV Longquan | KF294457.1 |
100 | 100 | 0 | 0 | Bat SARS CoV ZC45 | MG772933.1 | |
72 | 93,8 | 5* | 7* | Influenza A | MN319106.1 | |
54 | 100 | 2 | 10* | MERS CoV | MG987421.1 | |
T1_6—B1c (25 bp) | 100 | 100 | 0 | 0 | Bat SARS CoV Longquan | KF294457.1 |
100 | 100 | 0 | 0 | Bat SARS CoV ZC45 | MG772933.1 | |
52 | 100 | 5* | 12* | Influenza A | MK424175.1 | |
52 | 100 | 3* | 12* | Human adenovirus | KY002685.1 | |
48 | 100 | 5* | 13* | HCoV OC43 | OL770289.1 | |
T1_6—LB (24 bp) | 100 | 95,8 | 0 | 1 | Bat SARS CoV Longquan | KF294457.1 |
100 | 95,8 | 0 | 1 | Bat SARS CoV ZC45 | MG772933.1 | |
54 | 100 | 0 | 11* | Influenza A | MH592276.1 | |