Table 6 The results of primer hairpin analysis using RNAfold
Target | Primer | ΔG hairpin at 25 °C | ΔG hairpin at 60 °C |
|---|---|---|---|
T1_6 | F3 | − 0.16 | 0 |
B3 | − 1.30 | 0 | |
FIP | − 4.86 | 0 | |
BIP | − 4.05 | 0 | |
LB | − 3.00 | 0 | |
T1_9 | F3 | − 0.79 | 0 |
B3 | − 0.07 | 0 | |
FIP | − 4.66 | 0 | |
BIP | − 1.90 | 0 | |
LB | − 0.48 | 0 | |
T4_7 | F3 | − 2.09 | 0 |
B3 | − 1.22 | 0 | |
FIP | − 4.49 | − 0.47* | |
BIP | − 8.13 | − 1.41* | |
LB | − 0.36 | 0 | |
T4_52 | F3 | − 3.67 | 0 |
B3 | − 1.08 | 0 | |
FIP | − 5.68 | − 0.68* | |
BIP | − 3.49 | 0 |