Table 4 Results of homodimer analysis using oligoanalyzer
Target | Primer | ΔG Self dimer | ΔG hairpin | Tm hairpin oC |
|---|---|---|---|---|
T1_6 | F3 | − 5.4 | 0.4 | 18.4 |
B3 | − 9.3 | − 0.5 | 30.9 | |
FIP | − 8.4 | − 4.6 | 57.5 | |
BIP | − 7.6 | − 2.4 | 36.7 | |
LB | − 4.9 | − 1.1 | 35.6 | |
T1_9 | F3 | − 5.1 | − 0.5 | 30.6 |
B3 | − 3.6 | 0.3 | 16.9 | |
FIP | − 8.8 | − 3.5 | 50.4 | |
BIP | − 4.9 | − 1.1 | 31.1 | |
LB | − 3.9 | − 0.2 | 28.2 | |
T4_7 | F3 | − 7.1 | − 1.5 | 36.4 |
B3 | − 3.3 | − 0.7 | 35.8 | |
FIP | − 8.1 | − 3.3 | 42.7 | |
BIP | − 9.9 | − 6.1 | 52.1 | |
LB | − 5.4 | − 0.1 | 27.9 | |
T4_52 | F3 | − 3.9 | − 2.3 | 41.8 |
B3 | − 3.3 | − 1.1 | 38.1 | |
FIP | − 6.4 | − 4.0 | 64.7 | |
BIP | − 5.5 | − 3.0 | 39.7 |