Reverse transcription loop-mediated isothermal amplification (RT-LAMP) primer design based on Indonesia SARS-CoV-2 RNA sequence

Journal of Genetic Engineering and Biotechnology

Table 3 Best primer characteristic analysis results using Primer Explorer V5

Primer	Pjg. bases	% GC	Tm ^oC	ΔG 5'	ΔG 3'
T1_6-F3	21	48	61.0	− 5.4	− 5.9
T1_6-B3	21	48	58.8	− 4.4	− 4.6
T1_6-F2	24	42	59.7	− 5.9	− 5.0
T1_6-F1c	24	42	63.7	− 4.6	− 4.0
T1_6-B2	22	41	59.2	− 5.2	− 5.1
T1_6-B1c	25	44	63.6	− 6.0	− 5.9
T1_6-LB	24	42	62.5	− 4.2	− 4.4
T1_9-F3	23	43	58.6	− 4.9	− 4.5
T1_9-B3	22	45	59.1	− 5.6	− 4.5
T1_9-F2	22	41	58.1	− 4.7	− 4.8
T1_9-F1c	24	46	63.1	− 4.4	− 5.8
T1_9-B2	24	42	59.4	− 5.3	− 5.3
T1_9-B1c	25	44	63.3	− 6.2	− 6.2
T1_9-LB	23	43	61.5	− 4.0	− 4.9
T4_7-F3	23	43	60.8	− 5.0	− 4.2
T4_7-B3	22	50	60.3	− 5.2	− 5.4
T4_7-F2	23	43	60.6	− 7.1	− 4.9
T4_7-F1c	24	50	65.4	− 4.4	− 7.0
T4_7-B2	23	43	60.5	− 4.4	− 4.9
T4_7-B1c	25	52	65.4	− 4.7	− 5.6
T4_7-LB	24	46	60.8	− 4.8	− 5.9
T4_52-F3	23	43	60.0	− 4.8	− 4.1
T4_52-B3	21	48	60.9	− 4.0	− 6.4
T4_52-F2	25	40	60.8	− 4.1	− 4.3
T4_52-F1c	25	48	65.2	− 4.1	− 5.7
T4_52-B2	22	41	60.0	− 4.2	− 5.6
T4_52-B1c	24	50	65.4	− 4.0	− 5.8