Table 10 The results of the primer set specificity test T1_9 with the BLAST method
Primer | Query cover (%) | Identity (%) | Mismatch (bp) | Potential hybridization | Accession | |
|---|---|---|---|---|---|---|
End 3′ | Total | |||||
T1_9—F3 (23 bp) | 91 | 100 | 0 | 2 | Bat SARS CoV Longquan | KF294457.1 |
86 | 100 | 0 | 3 | Bat SARS CoV ZC45 | MG772933.1 | |
69 | 93,8 | 5* | 8* | Influenza A | KU160859.1 | |
52 | 100 | 5* | 11* | Human rubulavirus 2 | MH892405.1 | |
T1_9—B3 (22 bp) | 100 | 100 | 0 | 0 | Bat SARS CoV ZC45 | MG772933.1 |
95 | 90,5 | 1 | 3 | Bat SARS CoV Longquan | KF294457.1 | |
72 | 93,8 | 0 | 7* | MERS CoV | ON325306.1 | |
59 | 100 | 5* | 9* | Influenza A | L06576.1 | |
54 | 100 | 0 | 10* | Human metapneumovirus | MN745087.1 | |
T1_9—F2 (22 bp) | 100 | 95,5 | 1 | 1 | Bat SARS CoV ZC45 | MG772933.1 |
100 | 95,5 | 1 | 1 | Bat SARS CoV Longquan | KF294457.1 | |
72 | 93,8 | 5* | 7* | Influenza A | MW848655.1 | |
54 | 100 | 3* | 10* | Human adenovirus | KY002685.1 | |
T1_9—F1c (24 bp) | 66 | 93,8 | 1 | 9* | Influenza A | KF014012.1 |
58 | 100 | 5 | 10* | MERS CoV | MN481986.1 | |
54 | 100 | 4 | 11* | Human adenovirus | OL450401.1 | |
50 | 100 | 5* | 12* | HCoV OC43 | MN310478.1 | |
T1_9—B2 (24 bp) | 100 | 87,5 | 0 | 3 | Bat SARS CoV Longquan | KF294457.1 |
83 | 90 | 1 | 6 | Influenza A | OP023538.1 | |
50 | 100 | 4* | 12* | MERS CoV | MG596803.1 | |
50 | 100 | 5* | 12* | Human adenovirus | LC215437.1 | |
50 | 100 | 3* | 12* | Human metapneumovirus | LC671557.1 | |
T1_9—B1c (25 bp) | 100 | 92 | 0 | 2 | Bat SARS CoV ZC45 | MG772934.1 |
100 | 88 | 0 | 3 | Bat SARS CoV Longquan | KF294457.1 | |
64 | 93,8 | 5* | 10* | Human metapneumovirus | MH828686.1 | |
52 | 100 | 5* | 12* | Influenza A | MT624534.1 | |
48 | 100 | 5* | 13* | MERS CoV | MG987420.1 | |
T1_9 – LB (23 bp) | 69 | 93,8 | 5* | 8* | MERS CoV | MF593268.1 |
60 | 100 | 5* | 9* | Influenza A | EU980459.1 | |
52 | 100 | 5* | 11* | Bat SARS CoV ZC45 | MG772934.1 | |
52 | 100 | 5* | 11* | Human metapneumovirus | MH828687.1 | |