Table 1 Assembly statistics QUAST 4.0 and BUSCO 2.0
S.N | Assembly statistics | ABySS | SPAdes | Velvet |
|---|---|---|---|---|
1 | contigs (≥ 0 bp) | 376 | 7441 | 156 |
2 | contigs (≥ 500 bp) | 150 | 864 | 116 |
3 | contigs (≥ 1000 bp) | 124 | 122 | 96 |
4 | contigs (≥ 5000 bp) | 89 | 52 | 52 |
5 | contigs (≥ 10,000 bp) | 75 | 43 | 39 |
6 | contigs (≥ 25,000 bp) | 56 | 34 | 32 |
7 | contigs (≥ 50,000 bp) | 36 | 28 | 26 |
8 | Average no. of contigs/scaffold (bp) | 129.43 | 1226.285 | 73.85714 |
9 | Total length (≥ 0 bp) | 5,822,949 | 8,148,351 | 5,527,513 |
10 | Total length (≥ 500 bp) | 5,777,667 | 6,315,806 | 5,515,700 |
11 | Total length (≥ 1000 bp) | 5,759,070 | 5,832,559 | 5,501,478 |
12 | Total length (≥ 5000 bp) | 5,673,015 | 5,722,474 | 5,391,047 |
13 | Total length (≥ 10,000 bp) | 5,579,289 | 5,653,765 | 5,295,275 |
14 | Total length (≥ 25,000 bp) | 5,292,792 | 5,492,325 | 5,185,005 |
15 | Total length (≥ 50,000 bp) | 4,524,924 | 5,271,241 | 4,982,387 |
16 | Largest contigs | 537,434 | 732,848 | 698,773 |
17 | GC (%) | 35 | 38 | 35 |
18 | N50 | 116,621 | 202,477 | 200,267 |
19 | N75 | 61,283 | 94,390 | 137,847 |
20 | L50 | 15 | 9 | 8 |
21 | L75 | 33 | 21 | 16 |
22 | Ns per 100 kbp | 13.57 | 0 | 0 |
23 | Total BUSCO groups searched | 148 | 148 | 148 |
24 | Complete BUSCOs (C) | 147 | 147 | 147 |
25 | Complete and single-copy BUSCOs (S) | 144 | 143 | 144 |
26 | Complete and duplicated BUSCOs (D) | 3 | 4 | 3 |
27 | Fragmented BUSCOs (F) | 0 | 0 | 0 |
28 | Missing BUSCOs (M) | 1 | 1 | 1 |