Fig. 9
Metabolic pathway for PHA-producing Bacillus cereus BNPI-92 strain obtained from an area of plastic waste accumulation. A letter in green color box (a–v) indicates the most likely enzymes involved in metabolic activities. The letter in violet color box signifies possible gene involved in coding enzyme. List of enzymes: a not predicted in this study, b formate C-acetyltransferase [EC:2.3.1.54] (transferases), c acetyl-CoA C-acetyltransferase [EC:2.3.1.9] (transferases), d acetoacetyl-CoA reductase [EC:1.1.1.36] (oxidoreductases), e polyhydroxyalkanoate synthase subunit phaC [EC:2.3.1.-] (transferases), f butane monooxygenase alpha subunit [EC:1.14.13.230] (oxidoreductases), g butanol dehydrogenase [EC:1.1.1.-] (oxidoreductases), h acetaldehyde dehydrogenase (acetylating) [EC:1.2.1.10] (oxidoreductases), i butyryl-CoA dehydrogenase [EC:1.3.8.1] (oxidoreductases), k 3-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA-Delta-isomerase [EC:4.2.1.1205.3.3.3] (lyases), l butyrate kinase [EC:2.7.2.7] (transferases), m phosphate butyryltransferase [EC:2.3.1.19] (transferases), n 2-hydroxyglutarate dehydrogenase [EC:1.1.99.2], p glutaconate CoA-transferase, subunit A [EC:2.8.3.12] (transferases), q unidentified, r glutaconyl-CoA decarboxylase subunit alpha [EC:7.2.4.5], s 3-hydroxybutyryl-CoA dehydratase [EC:4.2.1.55] (lyases), t 3-hydroxybutyrate dehydrogenase [EC:1.1.1.30] (oxidoreductases), u hydroxybutyrate-dimer hydrolase [EC:3.1.1.22] (hydrolases), and v poly(3-hydroxybutyrate) depolymerase [EC:3.1.1.75] (hydrolases). List of few predicted gene in these pathways: a unidentified, b pf1D or it could be pflB [50] gene , c atoBgene, and d phbB gene. However, others were designated as phaA [51,52,53], orphbA [54, 55] gene, d its phaB [56, 57] or phbB [58] gene, e predicted as phaC or phbB gene , f unidentified in pathway, g it could be yugJ [59], h unidentified in the KEGG-KASS pathway, i gene encoding isologs of butyryl-CoA dehydrogenase enzyme are unidentified. It could be Swol_1933 [60], k croR gene, l unidentified in pathway, n unidentified gene, p it could be gctA , r it could be gcdA (s) croR gene, t it could be Bdh2 [61] gene, u unidentified, and v phaZ gene. These metabolic pathway and respective KEEG number database were collected from KEGG [62]