Fig. 8

PHA biosynthesis promoting genetic organizations (gene loci) in Bacillus sp. BNPI-92 and other closely related type strains genome ( Bacillus cereus ATCC 14579 T (AE016877), Bacillus paranthracis Mn5 T (MACE01000012), Bacillus thuringiensis ATCC 10792 T (ACNF01000156), and Bacillus antrics Amen T (AE016879). These strains harbored PHA biosynthesis gene with nearly similar nucleotide sequence size (nts) such as croR (predicted for PHA metabolisms), PhaQ (a PHB-responsive repressor controlling expression of phaP ), phaP (predicted for phasin biosynthesis), phaA (suggested for acetyle fermentation), phaB (predicted for butyrate fermentation), phaR (suggested as class IV PHA synthase or polyhydroxyalkanoate synthesis repressor phaR ), and phaC (predicted as PHA polymerization). phaA was located on a separate gene and suggested for encoding 3-ketoacyl-CoA thiolase or acetyl-CoA acetyltransferase protein. phaJ [5] (encoding enoyl-CoA hydratase) and acsA gene [49] (encoding acetoacetyl-CoA synthetase) predicted for PHA biosynthesis were located on the same operon. Isolog butyryl-CoA dehydrogenase enzyme is a gene which is unidentified