Fig. 7

Annotation overview and schematic representation of secondary metabolite gene clusters for PHA biosynthesis and a comparative PHA genetic organization for Bacillus sp. BNPI-92 and other closely related strains from RAST server annotation for a and b using online tools that are available at http://rast.theseed.org/FIG/rast.cgi. A deep red colored arrow indicates a phaC gene that is supposed to encode protein used for PHA polymerization. a is a phaA gene or nucleotide sequences (744 bp) encoded by acetyl-CoA thiolase and b is a phaC gene or nucleotide sequence (1086 bp) encoded by polyhydroxyalkanoic acid synthase. It is a conserved nucleotide sequence. And phaB gene (arrow with number 2) encodes acetoacetyl-CoA reductase. phaB and phaC genes are located on the same operon. Note that phaB gene sequence is not shown. c Three-dimensional structure of phaC protein with 319 residues (residue of protein structure for phaC gene) and presumed for PHA biosynthesis in Bacillus sp. BNPI-92. It has 319 residues. Its 88% has been modeled with 100.0% confidence by the single highest scoring template. The given protein residue resembles class I polyhydroxybutyrate synthase that was derived from Cupriavidus necator in terms of its structure. The three-dimensional structure of protein was predicted using PHYRE2 which is an online tool that is available at http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index (d). Evolutionary relationships for Bacillus sp. BNPI-92