Table 3 Concentrations of the key variables examined in the randomized Box–Behnken experiment and the observed responses of cholesterol degradation (%) and cholesterol oxidase activity (U/OD600) of B. subtilis
Trial | Cholesterol degradation | Cholesterol oxidase activity | ||||||
|---|---|---|---|---|---|---|---|---|
Cholesterol (g/l) | Inoculum size (%) | Magnesium sulphate (g/l) | Cholesterol degradation (%) | pH | Volume of the medium (ml/flask) | Cholesterol (g/l) | Cholesterol oxidase activity (U/OD600) | |
1 | 0.05 | 6 | 0.2 | 76 | 6 | 15 | 3.5 | 82.388 |
2 | 3.5 | 7 | 0.2 | 58 | 7 | 45 | 3.5 | 56.522 |
3 | 6.95 | 6 | 0.2 | 50 | 8 | 15 | 3.5 | 45.026 |
4 | 3.5 | 7 | 0.2 | 58 | 7 | 45 | 3.5 | 56.522 |
5 | 0.05 | 8 | 0.2 | 64 | 6 | 60 | 3.5 | 60.354 |
6 | 3.5 | 6 | 0.35 | 54 | 7 | 15 | 6.95 | 58.438 |
7 | 6.95 | 8 | 0.2 | 49 | 8 | 60 | 3.5 | 53.648 |
8 | 3.5 | 6 | 0.05 | 72 | 7 | 15 | 0.05 | 68.976 |
9 | 0.05 | 7 | 0.05 | 80 | 6 | 45 | 0.05 | 87.5612 |
10 | 0.05 | 7 | 0.35 | 58 | 6 | 45 | 6.95 | 63.228 |
11 | 6.95 | 7 | 0.05 | 53 | 8 | 45 | 0.05 | 57.48 |
12 | 6.95 | 7 | 0.35 | 47 | 8 | 45 | 6.95 | 41.8646 |
13 | 3.5 | 8 | 0.05 | 62 | 7 | 60 | 0.05 | 67.8264 |
14 | 3.5 | 8 | 0.35 | 51 | 7 | 60 | 6.95 | 51.732 |
15 | 3.5 | 7 | 0.2 | 59 | 7 | 45 | 3.5 | 55.564 |