Table 1 A randomized Plackett–Burman design applied on the degradation of cholesterol (%) and the activity of cholesterol oxidase (U/OD600) produced by B. subtilis
Trial | Independent variable | Dependent variable | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
C (g/l) | PP (g/l) | PN (g/l) | S (g/l) | M (g/l) | F (g/l) | V (ml/flask) | I (%) | pH | Cholesterol degradation (%) | Cholesterol oxidase activity (U/OD600) | |
1 | 6.0 | 0.125 | 1.5 | 0.5 | 0.0125 | 0.005 | 75 | 6.0 | 8 | 17.0 | 32.72 |
2 | 4.0 | 0.250 | 1.0 | 1.0 | 0.025 | 0.01 | 50.0 | 4.0 | 7.0 | 33.8 | 28.28 |
3 | 6.0 | 0.375 | 0.5 | 1.5 | 0.0125 | 0.005 | 25 | 6.0 | 8 | 15.4 | 67.48 |
4 | 2.0 | 0.375 | 0.5 | 0.5 | 0.0125 | 0.015 | 75 | 6.0 | 6 | 44.8 | 37.93 |
5 | 2.0 | 0.375 | 1.5 | 0.5 | 0.0375 | 0.005 | 25 | 2.0 | 8 | 35.0 | 27.32 |
6 | 2.0 | 0.125 | 0.5 | 1.5 | 0.0375 | 0.015 | 25 | 6.0 | 8 | 49.0 | 32.57 |
7 | 6.0 | 0.125 | 1.5 | 1.5 | 0.0125 | 0.015 | 25 | 2.0 | 6 | 15.3 | 68.03 |
8 | 2.0 | 0.375 | 1.5 | 1.5 | 0.0125 | 0.015 | 75 | 2.0 | 8 | 21.4 | 29.82 |
9 | 6.0 | 0.375 | 0.5 | 1.5 | 0.0375 | 0.005 | 75 | 2.0 | 6 | 19.2 | 66.45 |
10 | 6.0 | 0.375 | 1.5 | 0.5 | 0.0375 | 0.015 | 25 | 6.0 | 6 | 26.4 | 60.43 |
11 | 2.0 | 0.125 | 1.5 | 1.5 | 0.0375 | 0.005 | 75 | 6.0 | 6 | 41.7 | 33.18 |
12 | 6.0 | 0.125 | 0.5 | 0.5 | 0.0375 | 0.015 | 75 | 2.0 | 8 | 20.9 | 15.27 |
13 | 2.0 | 0.125 | 0.5 | 0.5 | 0.0125 | 0.005 | 25 | 2.0 | 6 | 36.1 | 61.87 |