Table 1 List of primers used
Primer’s name | Usability | Sequence 5′-3′ | Amplicon size (bp) |
|---|---|---|---|
RT-PCR L1 HPV-52 F | Stability test | TTG-TCG-ATT-CCA-GGC-TTC-CC | 96 |
RT-PCR L1 HPV-52 R | GCC-CCT-CGG-TGT-TTG-TAT-TT | ||
HPV-52 yeast F | Gene amplification | GAA-TTC-AAA-ACG-ATG-TCA-GTT-TGG-C | 1514 |
HPV-52 yeast R | CAA-ATG-TTC-GCA-TTC-TGA-CAT-CCT-CTT-GAG-C | ||
AOX1_forward | Analysis | GAC-TGG-TTC-CAA-TTG-ACA-AGC | - |
AOX1_reverse | Integration | GCA-AAT-GGC-ATT-CTG-ACA-TCC | - |