Table 2 Designed primers used in multiplex PCR
From: Colorimetric bacteria sensing of Pseudomonas aeruginosa using gold nanoparticle probes
Genes | Primers | Sequences (5′–3′) | Amplicon size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|
toxA | TOA-F | ACGCCCTGCATGTATCCTCCGA | 521 | 62 | |
TOA-R | CTTCGAGGCGGATGGTCAG | ||||
OPRL | OPL-F | TAGTGCTGGAAGGCCACACCGA | 256 | 62 | |
OPL-R | AGGAACGTCAGGACACGCAGGT | ||||
OPRI | OPI-F | AAACCGAAGCTCGTCTGACCGC | 172 | 62 | |
OPI-R | TTGCGGCTGGCTTTTTCCAGCA | ||||
16S rDNA | 16S-F | GGGGGATCTTCGGACCTCA | 956 | 62 | [31] |
16S-R | TCCTTAGAGTGCCCACCCG |