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Table 4 The effect of various concentrations of IBA and NAA in rooting of shoots on half-strength MS medium after 6 weeks

From: Control of media browning during micropropagation and assessment of biochemical and clonal fidelity of in vitro-derived and mother plants in Thottea siliquosa (Lamk.) Ding Hou., an important ethnomedicinal shrub

Auxin

Concentration (mg/L)

*Percentage of response

*Number of roots per shoot

*Root length (cm)

aExtent of callusing

IBA

0.00

0.00

0.00

0.0

 

0.25

22c

4.1 ± 0.34 cd

3.0 ± 0.34bc

-

0.5

63b

12.2 ± 0.5b

3.4 ± 0.48b

-

1.0

82a

20.8 ± 0.56a

4.8 ± 0.83a

-

1.5

60b

6.2 ± 0.32c

2.6 ± 0.34c

 + 

2.0

15de

2.16 ± 0.2de

2.46 ± 0.20 cd

 +  +  + 

NAA

0.25

10e

1.0 ± 0.24e

2.2 ± 0.34 cd

-

0.5

20c

1.5 ± 0.08de

3.1 ± 0.73bc

-

1.0

18 cd

2.3 ± 0.1d

3.2 ± 0.32b

 + 

1.5

16d

2.1 ± 0.23d

2.7 ± 0.19c

 +  +  + 

2.0

12ef

1.6 ± 0.16de

1.2 ± 0.13d

 +  +  +  + 

  1. *The values are represented as the means ± SE of three independent experiments. Means followed by the same letter within the column are not significantly different (p ≤ 0.05) according to Duncan’s multiple range test
  2. aThe increase in “ + ” sign shows a corresponding increase in callus induction