Table 3 Effect of various PGRs on callus induction and shoot regeneration on MS medium with 40Â mg/L ascorbic acid
Concentration of PGRs (mg/L) | *Percentage of response | *Fresh weight (mg) | *Dry weight (mg) | Shoot buds initiated per explant after 7Â weeks of culture | ||||
|---|---|---|---|---|---|---|---|---|
BA | KN | NAA | 2,4-D | *Percentage of response | *Number of shoots | |||
0.0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
0.25 |  | 0.5 |  | 9 g | 623 ± 1.68i | 64 ± 0.9f | 0.00 | 0.00 |
0.5 |  | 1.0 |  | 18ef | 782 ± 1.9f | 78 ± 0.7d | 0.00 | 0.00 |
0.75 |  | 1.5 |  | 27e | 846 ± 0.8c | 83 ± 0.2 cd | 67c | 4.0 ± 0.43c |
0.25 |  |  | 0.5 | 54c | 924 ± 0.5b | 90 ± 0.2ab | 69d | 9.6 ± 0.28b |
0.5 |  |  | 1.0 | 81a | 948 ± 0.8a | 93 ± 0.5a | 84a | 12.8 ± 0.87a |
0.75 |  |  | 1.5 | 66b | 928 ± 0.6b | 89 ± 0.4b | 81b | 6.1 ± 0.42bc |
0.25 | 0.5 |  | 16f | 765 ± 0.6 h | 72 ± 0.9ef | 0.00 | 0.00 | |
0.5 | 1.0 |  | 36d | 790 ± 1.7ef | 77 ± 0.5de | 0.00 | 0.00 | |
0.75 | 1.5 |  | 22ef | 774 ± 1.3 g | 73 ± 0.2e | 0.00 | 0.00 | |
0.25 |  | 0.5 | 25e | 795 ± 0.7e | 75 ± 0.3de | 29e | 2.3 ± 0.48d | |
0.5 |  | 1.0 | 56c | 816 ± 0.9d | 81 ± 0.4c | 34de | 1.8 ± 0.32d | |
0.75 |  | 1.5 | 34d | 802 ± 0.8de | 83 ± 0.8 cd | 47d | 1.2 ± 0.41e | |