Table 2 Effect of different concentrations and combinations of PGRs on direct shoot induction from cotyledon explants of T. siliquosa in WPM and MS media after 6 weeks of culture with 40 mg/L ascorbic acid

0.0

0.0

0.0

0.0

0.00

0.0

0.00

0.00

0.00

0.00

0.5

47f

38ef

3.7 ± 0.8d

2.0 ± 0.50ef

0.6 ± 1.24bc

1.0 ± 0.12b

1.0

58de

46e

6.2 ± 4.5bc

6.2 ± 0.4 cd

1.0 ± 0.56b

0.8 ± 0.22c

1.5

52e

22gh

5.3 ± 3.21 cd

4.5 ± 0.53d

0.7 ± 0.23bc

1.2 ± 0.1ab

2.0

17i

9ij

4.1 ± 14 cd

3.2 ± 0.23e

0.4 ± 0.43c

0.6 ± 0.04 cd

0.5

8j

3j

2.8 ± 0.23de

1.3 ± 0.46f

0.3 ± 0.28 cd

0.68 ± 0.23 cd

1.0

12i

6ij

2.0 ± 0.12e

2.0 ± 0.54ef

0.4 ± 0.12c

0.56 ± 0.56d

1.5

23 h

20gh

2.8 ± 0.17de

2.3 ± 0.40ef

0.2 ± 0.27 cd

1.1 ± 0.32b

2.0

20hi

12i

1.6 ± 0.14ef

1.2 ± 0.26f

1.1 ± 0.3b

0.7 ± 0.08 cd

0.5

56de

42ef

10.8 ± 4.42b

4.8 ± 0.23d

0.5 ± 0.32c

0.9 ± 0.03bc

1.0

72 cd

62c

12.6 ± 3.78ab

6.3 ± 0.34 cd

1.3 ± 0.32ab

1.1 ± 0.09b

1.5

64d

56d

8.5 ± 0.76bc

5.9 ± 0.38 cd

1.4 ± 0.19ab

0.9 ± 0.05bc

2.0

37 g

32f

4.0 ± 1.1d

2.0 ± 0.50ef

0.9 ± 0.48bc

1.0 ± 0.01b

1.0

0.1

75c

42ef

7.5 ± 0.18bc

9.2 ± 0.54c

1.0 ± 0.12b

0.9 ± 0.42bc

1.0

0.25

77bc

48e

8.7 ± 0.31bc

10.4 ± 0.67b

1.1 ± 0.72b

1.0 ± 0.96b

1.0

0.50

73 cd

34f

5.9 ± 0.63c

8.3 ± 0.34 cd

1.2 ± 0.42ab

1.2 ± 0.87ab

1.5

0.1

25 h

23 g

2.8 ± 0.03de

4.2 ± 0.32de

0.3 ± 0.23 cd

0.3 ± 0.34de

1.5

0.25

30gh

25 g

3.2 ± 0.14de

3.8 ± 0.12de

0.6 ± 0.67bc

0.5 ± 0.08d

1.5

0.50

28 h

20gh

4.7 ± 0.67 cd

2.8 ± 0.09ef

0.9 ± 0.25bc

0.8 ± 0.02bc

1.0

0.1

86b

72b

16.4 ± 0.41ab

10.3 ± 0.13b

1.3 ± 0.63ab

1.1 ± 0.47ab

1.0

0.25

92a

78a

20.9 ± 0.32a

12.9 ± 0.56a

1.6 ± 0.77a

1.4 ± 076a

1.0

0.50

84b

75b

16.4 ± 0.85b

10.6 ± 0.38b

1.6 ± 0.32a

1.2 ± 0.65ab