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Journal of Genetic Engineering and Biotechnology
Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Control of media browning during micropropagation and assessment of biochemical and clonal fidelity of in vitro-derived and mother plants in Thottea siliquosa (Lamk.) Ding Hou., an important ethnomedicinal shrub

Fig. 3

Callus-mediated shoot regeneration from cotyledon explants of T. siliquosa. a Callus formed at the cut end of cotyledon on MS medium supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L BA after 2 weeks (bar = 2 mm). b Seven-week-old green/brown, compact and nodular callus with minute shoot buds (arrow) (bar = 2 mm). c Differentiating callus on MS medium containing 0.5 mg/L BA and 0.25 mg/L NAA for shoot proliferation after 2 weeks (bar = 3 mm). d Same culture after 4 weeks. Shoots have elongated (bar = 5 mm). e Large number of well-developed multiple shoots differentiated from callus after 6 weeks (bar = 1 cm). f Histology of callus showing regenerated shoot bud (bar = 500 µm)

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