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Journal of Genetic Engineering and Biotechnology
Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Control of media browning during micropropagation and assessment of biochemical and clonal fidelity of in vitro-derived and mother plants in Thottea siliquosa (Lamk.) Ding Hou., an important ethnomedicinal shrub

Fig. 1

Direct shoot regeneration of T. siliquosa from cotyledon explants. a Germination of seeds (arrow) on MS basal medium after 1 week of culture (bar = 5 mm). b 7-day-old seedling with line of excision (dotted lines) of cotyledon (bar = 5 mm). c Cotyledon in the shoot induction medium consists of WPM with 1.0 mg/L TDZ and 0.25 mg/L NAA (bar = 2 mm). d Control culture (medium without ascorbic acid) showing browning due to phenolic exudation (bar = 8 mm). e Culture medium containing healthy shoots on optimum shoot induction medium with 40.0 mg/L ascorbic acid. There was no phenolic exudation (bar = 9 mm). f Shoot bud initiation (arrows) after 2 weeks of culture in the same medium as above. Shoot buds were originated from the explant (bar = 2.5 mm). g Same after 6 weeks of culture. Shoots elongated further (bar = 2.5 mm). h Subcultured shoots on WPM with 0.4 mg/L TDZ after 4 weeks. Several elongated shoots are visible (bar = 1 cm). i Histological analysis of direct shoot organogenesis showing multiple shoots with vascular connection (arrow) from the explant. SAM, shoot apical meristem; VC, vascular connection (bar = 500 µm)

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